Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL(SPHERO™彩虹校准微球 )

Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL(SPHERO™彩虹校准微球 )
货号:RCP-30-5A
品名:Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL(SPHERO™彩虹校准微球 )
品牌:Spherotech
规格:5mL
价格:请询价
产品详情介绍
一、产品描述
品牌:Spherotech
货号:RCP-30-5A
英文名:SPHERO™ Rainbow Calibration Particles, 8 Peaks, 3.0-3.4 μm
中文名:SPHERO™彩虹校准微球
规格:5mL
SPHERO™彩虹校准微球是Spherotech公司专为流式细胞仪校准设计的高精度荧光微球产品。其核心成分为聚苯乙烯微球,粒径均一,内部均匀嵌入8种不同荧光强度的荧光染料混合物,覆盖FITC、PE、PE-Cy5、APC等主流荧光通道的激发波长。该产品以即用型滴管瓶封装,支持直接稀释后上机使用,适用于流式细胞仪的光路校准、灵敏度验证、线性度评估及长期性能跟踪。
二、产品特点
1. 多通道同步校准
单一微球可同时校准流式细胞仪的前向散射(FSC)、侧向散射(SSC)及8个荧光通道,通过检测荧光峰的位置和强度,验证仪器灵敏度、线性度和分辨率。
2. 荧光稳定性设计
荧光染料通过物理包埋技术固定在微球内部,避免外界环境对信号的干扰,确保长期储存和重复使用时的信号一致性。
3. 对数放大器线性验证
微球荧光强度呈对数分布,可通过配套Excel模板生成校准曲线,定量评估仪器对数放大器的线性误差,确保检测结果的准确性。
4. 高兼容性与经济性
• 适配主流品牌的流式细胞仪及荧光显微镜、共聚焦显微镜等设备。
• 每瓶可进行约50-100次校准,单次实验成本显著低于同类产品。
5. 技术支持与资源
提供详细技术网页和操作视频,指导用户完成校准流程及数据分析。
三、储存条件
• 温度:2-8℃避光保存,避免冷冻。
• 有效期:未开封状态下12个月,开封后建议6个月内使用完毕。
• 注意事项:
• 使用前需充分混匀微球悬液,避免沉淀导致浓度不均。
• 校准过程中需使用过滤后的去离子水或鞘液,防止杂质干扰检测结果。
四、工作原理
1. 多色荧光标记技术
微球内部嵌入8种荧光染料,激发波长覆盖365-650nm,可适配流式细胞仪的多种激光系统。通过检测各通道的荧光信号强度,验证仪器对不同波长光的响应能力。
2. 粒径与荧光强度梯度设计
微球粒径均一(CV<2%),荧光强度呈梯度分布,用于评估仪器在不同信号强度下的线性响应和分辨率。
3. 对数放大器校准
荧光信号强度呈对数分布,通过分析各峰的信号值,生成对数放大器校准曲线,修正仪器非线性误差。
五、解决实验中的关键问题
1. 仪器标准化与质量控制
• 光路校准:调整液流稳定性和激光对齐,确保细胞大小和颗粒度检测的准确性。
• 荧光通道灵敏度验证:检测微球荧光强度阈值,确认仪器对弱信号的检测能力。
• 线性度评估:验证对数放大器在宽动态范围内的线性响应。
2. 实验数据可比性与重复性
• 多实验室数据标准化:统一使用RCP-30-5A可消除仪器差异,实现跨平台数据可比性。
• 长期性能跟踪:定期校准可监测仪器性能变化,避免系统性偏差。
3. 复杂实验优化
• 荧光补偿设置:通过多色荧光特性快速建立补偿矩阵,减少光谱重叠干扰。
• 多色通道匹配:根据微球荧光发射谱选择染料组合,提高检测维度。
六、使用方法
1. 样品准备
• 取1-2滴微球悬液,用过滤后的去离子水或鞘液稀释至适宜浓度。
2. 仪器设置
• 选择与微球荧光染料匹配的激光器和滤光片组合。
• 设置前向散射(FSC)和侧向散射(SSC)参数,优化液流稳定性。
3. 校准执行
• 采集至少10,000个微球事件,记录各通道的荧光信号分布。
• 使用配套Excel模板分析数据,生成校准曲线并调整仪器参数。
4. 数据分析
• 验证各通道的灵敏度、线性度和分辨率,确保符合实验要求。
• 记录校准结果,用于后续实验的质量控制参考。
5. 校准后处理
• 清洁流式细胞仪管路,避免微球残留影响后续实验。
七、常见问题与解决方案
• 问题1:荧光信号强度异常
原因:激光器功率波动或微球批次差异。
解决方案:重新校准激光器电压,更换新批次微球。
• 问题2:CV值偏高
原因:液流不稳定或微球沉降。
解决方案:优化鞘液压力,使用前充分混匀微球悬液。
• 问题3:补偿矩阵计算错误
原因:荧光染料光谱重叠或数据采集参数设置不当。
解决方案:使用单染色对照重新计算补偿矩阵,调整检测电压。
• 问题4:微球堵塞管路
原因:微球粒径过大或浓度过高。
解决方案:稀释微球悬液至推荐浓度,检查流式细胞仪管路清洁度。
八、免责声明
1. 本产品需在专业人员指导下使用,因操作不当导致的仪器损坏或数据偏差,Spherotech不承担直接责任。
2. 储存条件不符合要求(如高温、冷冻)可能影响产品性能,用户需自行承担相关风险。
3. 本产品仅用于科研及工业质检,不可用于临床诊断或治疗。
4. 技术支持仅限于产品性能问题,不涉及用户实验设计或数据分析的最终结果。
5. 本文内容基于品牌公开资料及行业常规信息整理,仅个人观点,具体以品牌信息文档为准。
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Spherotech |
|
TFP-0556-5 |
Biotin Fluorescent Particles, Nile Red, 0.1% w/v, 0.4-0.6 um, 5 mL |
5mL |
Spherotech |
|
PP-08-10 |
polystyrene particles,5%w/v,0.7-0.9um |
10ml |
Spherotech |
|
CM-200-10 |
Carboxyl Magnetic Particles, 1%w/v, 18.0-22.9µm, 10mL |
10mL |
Spherotech |
|
NFPPS-1552-5 |
Nano Fluorescent Size Standard, Yellow, Flow Cytometry Grade,1.0-1.9 μm |
5ml |
Spherotech |
|
CM-05-10H |
Carboxyl Magnetic Particles, High Iron, 1.0%w/v, 0.4-0.69µm, 10mL |
10mL |
Spherotech |
|
PP-008-10 |
POLYSTYRENE PARTICLES |
10ml |
Spherotech |
|
PGP-20-5 |
Protein G Polystyrene Particles, 0.5%w/v, 2.0-2.9µm, 5mL |
5mL |
Spherotech |
