Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)

Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)
货号:16-16-090707-1M
品名:Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)
品牌:Athens Research
规格:1mg
价格:请询价
产品详情介绍
产品描述
品牌:ATHENS RESEARCH & TECHNOLOGY
货号:16-16-090707-1M
品名:免疫球蛋白G1(IgG1),人骨髓瘤血浆
规格:1 mg
英文名:Immunoglobulin G1 (IgG1), Human Myeloma Plasma
中文名:免疫球蛋白G1(IgG1),人骨髓瘤血浆
本产品为从人骨髓瘤血浆中纯化的免疫球蛋白G1(IgG1),适用于生命科学研究领域。IgG1是免疫球蛋白G(IgG)的主要亚类之一,在体液免疫中发挥核心作用,包括抗原中和、补体激活及免疫细胞信号传导。本产品通过严格的层析纯化技术制备,纯度≥95%,不含HBsAg、HCV、HIV等病原体污染,符合FDA生物安全标准。其分子量为约50 kDa,溶解于pH 7.4的磷酸盐缓冲液中,适用于ELISA、Western blotting、免疫组化、流式细胞术等多种实验场景。
产品特点
1. 高纯度与稳定性:采用离子交换层析和亲和层析技术,确保IgG1的纯度≥95%,且长期保存(-20°C)下稳定性优异,无聚集或降解现象。
2. 功能完整性:保留完整的Fc段结构,可有效结合Fc受体及补体成分,支持下游功能实验。
3. 低背景干扰:经严格去内毒素处理,适用于敏感实验。
4. 广泛兼容性:适用于哺乳动物细胞系及原代细胞,兼容多种缓冲体系。
储存条件
• 短期储存:2-8°C(建议不超过1个月)。
• 长期储存:-20°C(避免反复冻融,建议分装保存)。
• 运输条件:干冰运输,确保全程低温。
工作原理
IgG1通过其可变区(Fab)特异性识别并结合抗原表位,Fc段则与免疫效应细胞表面的Fcγ受体结合,触发吞噬作用或细胞毒性反应。此外,IgG1可激活补体系统,介导病原体裂解或靶细胞溶解。在实验中,其应用场景包括:
• ELISA:作为捕获抗体或检测抗体,用于抗原定量分析。
• Western blotting:识别特定蛋白条带,验证蛋白表达水平。
• 免疫组化:标记组织切片中的目标抗原,定位蛋白分布。
解决实验中哪些问题
1. 标准化问题:提供高纯度IgG1,减少实验批次间的差异,确保结果可重复性。
2. 信号弱:通过优化抗体浓度,增强检测灵敏度。
3. 非特异性结合:预吸附处理可降低背景信号,提升信噪比。
4. 细胞毒性:低内毒素特性适用于活细胞实验,避免干扰细胞活性。
使用方法
1. 实验前准备:
• 从-20°C取出产品,置于冰上解冻,避免反复冻融。
• 根据实验需求稀释抗体。
2. ELISA应用:
• 包被:将稀释后的IgG1加入预包被孔板,4°C孵育过夜。
• 洗涤:用TBST缓冲液洗板3次,每次5分钟。
• 封闭:加入5% BSA溶液,室温孵育1小时。
3. Western Blotting:
• 电泳后转膜,封闭后加入一抗,4°C孵育过夜。
• 二抗孵育,室温1小时,ECL显影。
4. 免疫组化:
• 组织切片脱蜡后,抗原修复。
• 滴加IgG1工作液,湿盒内37°C孵育1小时,DAB显色。
常见问题与解决方案
• 问题1:实验信号弱或无信号
原因:抗体浓度过低、抗原表位遮蔽或样本降解。
解决方案:优化抗体浓度,验证样本完整性。
• 问题2:高背景干扰
原因:封闭不充分或抗体非特异性结合。
解决方案:延长封闭时间,使用更高效封闭剂。
• 问题3:储存后抗体沉淀
原因:反复冻融或储存温度波动。
解决方案:分装保存(每份≤100 μL),避免温度剧烈变化。
• 问题4:与Fc受体非特异性结合
原因:Fc段与细胞表面受体交联。
解决方案:使用Fc段封闭剂预处理样本。
免责声明
1. 本产品为科研用途试剂,不可用于临床诊断或治疗。
2. 使用前需进行预实验验证,具体实验条件需根据样本特性调整。
3. 产品性能可能因储存不当或操作失误而受影响,建议严格遵循说明书。
4. ATHENS RESEARCH & TECHNOLOGY 不对因产品使用不当导致的实验失败承担责任。
5. 本文内容基于品牌公开资料及行业常规信息整理,仅个人观点,具体以品牌信息文档为准。
⚡ATHENS RESEARCH热卖产品
|
货号 |
品名 |
规格 |
品牌 |
|
16-16-090707-1M |
Immunoglobulin G1, Human Myeloma Plasma |
1mg |
athensresearch |
|
16-16-090707-2M-1MG |
Immunoglobulin G2 Human Myeloma Plasma |
1mg |
athensresearch |
|
16-16-090705-100ug |
Immunoglobulin E, Human Plasma |
100ug |
athensresearch |
|
16-16-090701-1M-10mg |
Immunoglobulin A1, Human Myeloma Plasma |
10mg |
athensresearch |
|
16-14-051200-100ug |
Elastase, Human Neutrophil |
100ug |
athensresearch |
|
16-16-032001-LEL-1gram |
Transferrin (HOLO), Human Plasma, Tissue Culture Grade |
1gram |
athensresearch |
|
16-16-161213-L-1mg |
Plasmin, Human Plasma (lyophilized) |
1mg |
athensresearch |
|
16-16-120303-1mg |
Apolipoprotein CIII, Human Plasma |
1mg |
athensresearch |
|
12-16-121601-1mg |
Lipoprotein (a), Human Plasma |
1mg |
athensresearch |
|
16-16-090701-1M-1mg |
Immunoglobulin A1, Human Myeloma Plasma |
1mg |
athensresearch |
|
12-16-121601-100ug |
Lipoprotein (a), Human Plasma |
100ug |
athensresearch |
|
16-13-090701-5mg |
Secretory IgA, Human Colostrum |
5mg |
athensresearch |
|
16-14-012621-1mg |
Azurocidin, Human Neutrophil |
1mg |
athensresearch |
|
16-14-130000-100ug |
Myeloperoxidase, Human Neutrophil |
100ug |
athensresearch |
|
16-14-130000-1mg |
Myeloperoxidase, Human Neutrophil |
1mg |
athensresearch |
|
16-16-080116-1/1-1mg |
Haptoglobin, Phenotype 1-1, Human Plasma |
1mg |
athensresearch |
|
16-16-090701-1M-5mg |
Immunoglobulin A1, Human Myeloma Plasma |
5mg |
athensresearch |
|
16-16-090707-1M-10mg |
Immunoglobulin G1, Human Myeloma Plasma |
10mg |
athensresearch |
|
16-16-090713-10mg |
Immunoglobulin M, Human Plasma |
10mg |
athensresearch |
|
16-16-090713-1mg |
Immunoglobulin M, Human Plasma |
1mg |
athensresearch |
|
16-16-090713-M-1MG |
Immunoglobulin M (IgM) Human Myeloma Plasma |
1mg |
athensresearch |
|
16-12-030102-25ug |
Cathepsin B, Human Liver |
25ug |
athensresearch |
|
16-12-030102-50ug |
Cathepsin B, Human Liver |
50ug |
athensresearch |
|
16-12-030104-100ug |
Cathepsin D, Human Liver |
100ug |
athensresearch |
|
16-12-030112-25ug |
Cathepsin L, Human Liver |
25ug |
athensresearch |
|
16-13-090701-1mg |
Secretory IgA, Human Colostrum |
1mg |
athensresearch |
|
16-14-030107-50ug |
Cathepsin G, Human Neutrophil |
50ug |
athensresearch |
|
16-14-051200-50ug |
Elastase |
50 ug |
athensresearch |
|
16-14-161820-100ug |
Proteinase 3, Human Neutrophil |
100ug |
athensresearch |
|
16-16-011609-1mg |
Alpha 1 Antitrypsin, Human Plasma |
1mg |
athensresearch |
|
16-16-011609-5mg |
Alpha 1 Antitrypsin, Human Plasma |
5mg |
athensresearch |
|
16-16-012020-1mg |
Antithrombin III, Human Plasma |
1mg |
athensresearch |
|
16-16-032001-CM-5mg |
Transferrin, Cynomolgus Monkey |
5 mg |
athensresearch |
|
16-16-080116-1mg |
Haptoglobin, Human Plasma, Mixed Type |
1mg |
athensresearch |
|
16-16-080116-2/2-1mg |
Haptoglobin, Phenotype 2-2, Human Plasma |
1mg |
athensresearch |
|
16-16-080116-5mg |
Haptoglobin, Human Plasma, Mixed Type |
5mg |
athensresearch |
|
16-16-090701-10mg |
Immunoglobulin A1 (IgA1), Human Myeloma Plasma |
10mg |
athensresearch |
|
16-16-090701-2M-1mg |
Immunoglobulin A2, Human Plasma |
1mg |
athensresearch |
|
16-16-090704-0.1mg |
免疫球蛋白D (IgD) |
0.1mg |
athensresearch |
|
16-16-090707-FC-1mg |
Immunoglobulin G, FC Fragment, Human Plasma |
1mg |
athensresearch |
|
16-16-090713-Mfc5u-1mg |
Immunoglobulin M, Fc5mu, Human Plasma |
1mg |
athensresearch |
|
16-16-120101-500ug |
Apolipoprotein AI, Human Plasma |
500ug |
athensresearch |
|
16-16-161200-1mg |
Plasminogen, Human Plasma |
1mg |
athensresearch |
|
16-16-161213-F |
Plasmin, Frozen |
1mg |
athensresearch |
|
01-05-030000-1ML |
Anti-Catalase Polyclonal Ab, Human Erthrocyte |
1ml |
athensresearch |
|
12-16-030825-1mg |
Chylomicrons, Human Plasma |
1mg |
athensresearch |
|
12-16-120412-5mg |
Lipoproteins, Low Density, Human Plasma |
5mg |
athensresearch |
|
12-16-120412-OX-10mg |
脂蛋白, 低密度, 氧化 (LDL-OX) |
10mg |
athensresearch |
|
12-16-120412-OX-1mg |
Lipoproteins, Oxidized, Low Density, Human Plasma |
1mg |
athensresearch |
|
12-16-120412-OX-5mg |
脂蛋白, 低密度, 氧化 (LDL-OX) |
5mg |
athensresearch |
|
12-16-221204-5mg |
Lipoproteins, Very Low Density, Human Plasma |
5mg |
athensresearch |
|
16-05-030000-1mg |
Catalase, Human Erythrocyte (50 KU per mg) |
1mg |
athensresearch |
|
16-05-030112-P-100ug |
Calpain-1, Porcine Erythrocytes |
100 ug |
athensresearch |
|
16-05-030112-P-1mg |
Calpain-1, Porcine Erythrocytes |
1 mg |
athensresearch |
|
16-12-030104-25ug |
Cathepsin D, Human Liver |
25ug |
athensresearch |
|
16-13-011202 |
Lactalbumin |
5 mg |
athensresearch |
|
16-13-090701-10mg |
Secretory IgA, Human Colostrum |
10mg |
athensresearch |
|
16-13-A20103 |
Apolactoferrin, Human Milk |
5mg |
athensresearch |
|
16-14-012621-100ug |
Azurocidin, Human Neutrophil |
100ug |
athensresearch |
|
16-14-030107-100ug |
Cathepsin G, Human Neutrophil |
100ug |
athensresearch |
|
16-14-051200-1mg |
Elastase, Human Neutrophil |
1mg |
athensresearch |
|
16-14-161820-25ug |
Proteinase 3 (PR3), Human Neutrophil |
25ug |
athensresearch |
|
16-15-160518-100ug |
Eosinophil Peroxidase, Human Eosinophils |
100ug |
athensresearch |
|
16-16-010700-10mg |
Alpha 1 Acid Glycoprotein (A1AGP), Human Plasma |
10mg |
athensresearch |
|
16-16-010700-1mg |
α1酸性糖蛋白 (A1AGP) |
1mg |
athensresearch |
|
16-16-011202-CM-10mg |
Albumin, Cynomolgus Monkey ** |
10mg |
athensresearch |
|
16-16-012013-1mg |
Alpha 2 Macroglobulin, Human Plasma |
1mg |
athensresearch |
|
16-16-012400-1mg |
Alpha 1 Antichymotrypsin, Human Plasma |
1mg |
athensresearch |
|
16-16-012901-100UG |
Alpha 2 Antiplasmin, Human Plasma |
100ug |
athensresearch |
|
16-16-012901-1MG |
Alpha 2 Antiplasmin, Human Plasma |
1mg |
athensresearch |
|
16-16-012901-500UG |
Alpha 2 Antiplasmin, Human Plasma |
500ug |
athensresearch |
|
16-16-030402-100ug |
C4b-Binding Protein, Human Plasma |
100ug |
athensresearch |
|
16-16-030402-1mg |
C4b-Binding Protein, Human Plasma |
1mg |
athensresearch |
|
16-16-030518-1mg |
Ceruloplasmin, Human Plasma |
1mg |
athensresearch |
|
16-16-032001-1gram |
Transferrin (HOLO), Human Plasma |
1 gram |
athensresearch |
|
16-16-032001-CM-1mg |
Transferrin, Cynomolgus Monkey ** |
1mg |
athensresearch |
|
16-16-080513-LEL |
Hemopexin, Low Endotoxin Level, Human Plasma |
1mg |
athensresearch |
|
16-16-081907-1mg |
Alpha 2 HS Glycoprotein, Human Plasma |
1mg |
athensresearch |
|
16-16-090109-100ug |
Inter Alpha Inhibitor Proteins (IaIp), Human Plasma |
100ug |
athensresearch |
|
16-16-090109-1mg |
Inter Alpha Inhibitor Proteins (IaIp), Human Plasma |
1mg |
athensresearch |
|
16-16-090109-25ug |
Inter Alpha Inhibitor Proteins (IaIp), Human Plasma |
25ug |
athensresearch |
|
16-16-090701-1mg |
Immunoglobulin A, Human Plasma |
1mg |
athensresearch |
|
16-16-090705 |
Immunoglobulin E, Human Plasma 1mg |
1mg |
athensresearch |
|
16-16-090705-M-Lambda-100ug |
Immunoglobulin E, Human Myeloma Plasma, Lambda |
100ug |
athensresearch |
|
16-16-090707 |
人免疫球蛋白 |
毫克 |
athensresearch |
|
16-16-090707-1-1mg |
Immunoglobulin G1, Human Normal Plasma |
1mg |
athensresearch |
|
16-16-090707-1M-5mg |
Immunoglobulin G1, Human Myeloma Plasma |
5mg |
athensresearch |
|
16-16-090707-2M-10mg |
Immunoglobulin G2, Human Myeloma Plasma |
10mg |
athensresearch |
|
16-16-090707-2M-5mg |
Immunoglobulin G2, Human Myeloma Plasma |
5mg |
athensresearch |
|
16-16-090707-4M-1mg |
Immunoglobulin G4, Human Myeloma Plasma |
1mg |
athensresearch |
|
16-16-090707-FAB2 |
Immunoglobulin G (IgG), Fab '2 |
1 mg |
athensresearch |
|
16-16-090707-Fc-10mg |
Immunoglobulin G, Fc Fragment, Human Plasma |
10mg |
athensresearch |
|
16-16-090707-mouse |
Immunoglobulin G (IgG), Mouse |
5 mg |
athensresearch |
|
16-16-090713-BULK |
Immunoglobulin M, Human Plasma |
1g |
athensresearch |
|
16-16-090713-Mfc5u-5mg |
免疫球蛋白M Fc5mu |
5mg |
athensresearch |
|
16-16-110112-50ug |
Kallikrein, Human Plasma (frozen) |
50ug |
athensresearch |
|
16-16-110112-CM |
Kallikrein, Cynomolgus Monkey Plasma |
50ug |
athensresearch |
|
16-16-110112-RAT-50ug |
Kallikrein, Rat Plasma |
50ug |
athensresearch |
|
16-16-110914-L-1mg |
Kininogen, Low Molecular Weight, Human Plasma |
1mg |
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