13530144996

Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)

9/27/2026 4:47:00 PM

Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)

货号:16-16-090707-1M

品名:Immunoglobulin G1, Human Myeloma Plasma(免疫球蛋白G1,人骨髓瘤血浆)

品牌:Athens Research

规格:1mg

价格:请询价

 

产品详情介绍

产品描述

品牌:ATHENS RESEARCH & TECHNOLOGY  

货号:16-16-090707-1M  

品名:免疫球蛋白G1(IgG1),人骨髓瘤血浆  

规格:1 mg  

英文名:Immunoglobulin G1 (IgG1), Human Myeloma Plasma  

中文名:免疫球蛋白G1(IgG1),人骨髓瘤血浆  

本产品为从人骨髓瘤血浆中纯化的免疫球蛋白G1(IgG1),适用于生命科学研究领域。IgG1是免疫球蛋白G(IgG)的主要亚类之一,在体液免疫中发挥核心作用,包括抗原中和、补体激活及免疫细胞信号传导。本产品通过严格的层析纯化技术制备,纯度≥95%,不含HBsAg、HCV、HIV等病原体污染,符合FDA生物安全标准。其分子量为约50 kDa,溶解于pH 7.4的磷酸盐缓冲液中,适用于ELISA、Western blotting、免疫组化、流式细胞术等多种实验场景。  

 

产品特点

1. 高纯度与稳定性:采用离子交换层析和亲和层析技术,确保IgG1的纯度≥95%,且长期保存(-20°C)下稳定性优异,无聚集或降解现象。  

2. 功能完整性:保留完整的Fc段结构,可有效结合Fc受体及补体成分,支持下游功能实验。  

3. 低背景干扰:经严格去内毒素处理,适用于敏感实验。  

4. 广泛兼容性:适用于哺乳动物细胞系及原代细胞,兼容多种缓冲体系。  

 

储存条件

• 短期储存:2-8°C(建议不超过1个月)。  

• 长期储存:-20°C(避免反复冻融,建议分装保存)。  

• 运输条件:干冰运输,确保全程低温。  

 

工作原理

IgG1通过其可变区(Fab)特异性识别并结合抗原表位,Fc段则与免疫效应细胞表面的Fcγ受体结合,触发吞噬作用或细胞毒性反应。此外,IgG1可激活补体系统,介导病原体裂解或靶细胞溶解。在实验中,其应用场景包括:  

• ELISA:作为捕获抗体或检测抗体,用于抗原定量分析。  

• Western blotting:识别特定蛋白条带,验证蛋白表达水平。  

• 免疫组化:标记组织切片中的目标抗原,定位蛋白分布。  

 

解决实验中哪些问题

1. 标准化问题:提供高纯度IgG1,减少实验批次间的差异,确保结果可重复性。  

2. 信号弱:通过优化抗体浓度,增强检测灵敏度。  

3. 非特异性结合:预吸附处理可降低背景信号,提升信噪比。  

4. 细胞毒性:低内毒素特性适用于活细胞实验,避免干扰细胞活性。  

 

使用方法

1. 实验前准备:  

   • 从-20°C取出产品,置于冰上解冻,避免反复冻融。  

   • 根据实验需求稀释抗体。  

2. ELISA应用:  

   • 包被:将稀释后的IgG1加入预包被孔板,4°C孵育过夜。  

   • 洗涤:用TBST缓冲液洗板3次,每次5分钟。  

   • 封闭:加入5% BSA溶液,室温孵育1小时。  

3. Western Blotting:  

   • 电泳后转膜,封闭后加入一抗,4°C孵育过夜。  

   • 二抗孵育,室温1小时,ECL显影。  

4. 免疫组化:  

   • 组织切片脱蜡后,抗原修复。  

   • 滴加IgG1工作液,湿盒内37°C孵育1小时,DAB显色。  

 

​常见问题与解决方案

• 问题1:实验信号弱或无信号  

  原因:抗体浓度过低、抗原表位遮蔽或样本降解。  

  解决方案:优化抗体浓度,验证样本完整性。  

• 问题2:高背景干扰  

  原因:封闭不充分或抗体非特异性结合。  

  解决方案:延长封闭时间,使用更高效封闭剂。  

• 问题3:储存后抗体沉淀  

  原因:反复冻融或储存温度波动。  

  解决方案:分装保存(每份≤100 μL),避免温度剧烈变化。  

• 问题4:与Fc受体非特异性结合  

  原因:Fc段与细胞表面受体交联。  

  解决方案:使用Fc段封闭剂预处理样本。  

 

免责声明

1. 本产品为科研用途试剂,不可用于临床诊断或治疗。  

2. 使用前需进行预实验验证,具体实验条件需根据样本特性调整。  

3. 产品性能可能因储存不当或操作失误而受影响,建议严格遵循说明书。  

4. ATHENS RESEARCH & TECHNOLOGY 不对因产品使用不当导致的实验失败承担责任。  

5. 本文内容基于品牌公开资料及行业常规信息整理,仅个人观点,具体以品牌信息文档为准。

 

 

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