PureCol®, Bovine Collagen, 3 mg/mL, 100 mL（牛胶原蛋白）

9/27/2026 4:55:00 PM

PureCol®, Bovine Collagen, 3 mg/mL, 100 mL（牛胶原蛋白）

货号：5005-100ML

品名：PureCol®, Bovine Collagen, 3 mg/mL, 100 mL（牛胶原蛋白）

品牌：Advanced BioMatrix

规格：100ml

价格：请询价

产品详情介绍

产品描述

货号：5005-100ML

英文名：PureCol®, Bovine Collagen, 3 mg/mL, 100 mL

中文名：牛去端肽胶原溶液（I型，3 mg/mL，100 mL）

品牌：Advanced BioMatrix

规格：100 mL

成分：牛源I型胶原蛋白（占比约97%）、III型胶原蛋白（占比约3%），溶于0.01 M HCl（pH 2.0），含无菌过滤的DMEM/F-12培养基及L-谷氨酰胺。

特性：高纯度（≥99.9%胶原蛋白含量）、成分明确、预配即用型溶液，适用于2D/3D细胞培养、组织工程及生物打印。

产品特点

1. 高纯度与一致性：采用封闭式牛群原料及cGMP生产工艺，确保批次间稳定性，纯度经SDS-PAGE银染验证≥99.9%。

2. 即用型设计：无需预混或pH调整，直接用于细胞涂覆或凝胶制备，简化实验流程。

3. 生物相容性：支持多种细胞类型的黏附与增殖，模拟天然细胞外基质（ECM）微环境。

4. 广泛适用性：兼容2D平面涂层、3D水凝胶构建及生物打印，适用于药物筛选、创伤修复及组织再生研究。

储存条件

• 温度：2–10°C冷藏，避免冷冻或反复冻融。

• 避光：需避光保存以维持胶原活性。

• 保质期：自生产之日起24个月（具体以标签或分析证书为准）。

工作原理

PureCol®胶原通过提供三维网状结构模拟天然ECM，其I型胶原的三螺旋构象可促进细胞黏附、迁移及信号传导。在3D环境中，胶原凝胶的刚度（弹性模量）可调控细胞行为，例如：

• 机械信号传导：高刚度基质诱导成纤维细胞收缩表型，低刚度促进神经突触延伸。

• 细胞-基质相互作用：整合素受体（如α1β1、α2β1）与胶原三螺旋结合，激活下游信号通路（如FAK、MAPK）。

解决实验中的关键问题

1. 3D培养模型构建：替代传统2D培养，更真实模拟体内微环境，用于研究肿瘤转移、组织再生等。

2. 药物筛选平台：通过调节胶原凝胶刚度，评估药物对细胞迁移、侵袭及凋亡的影响。

3. 创伤修复研究：模拟皮肤或角膜创伤的胶原支架，支持细胞迁移与组织再生。

4. 生物打印应用：作为生物墨水，结合光交联技术构建复杂组织结构。

使用方法

1. 2D涂层

• 步骤：

• 解冻胶原溶液至4°C（避免反复冻融）。

• 稀释至50–100 μg/mL（推荐比例1:30，用0.01 M HCl或PBS）。

• 均匀涂覆于培养皿/孔板表面，室温孵育1–2小时。

• 弃去多余溶液，用无菌培养基冲洗表面。

• 注意事项：涂覆后需保持湿润，避免干燥。

2. 3D凝胶制备

• 步骤：

• 混合胶原溶液与10×PBS或培养基（体积比8:1），缓慢加入中和剂（如0.1 M NaOH）调节pH至7.0–7.5。

• 预冷至2–10°C，避免提前凝胶化。

• 37°C孵育90–120分钟形成凝胶。

• 应用：可直接接种细胞，研究3D迁移、药物渗透等。

常见问题与解决方案

• 问题1：凝胶形成失败

• 原因：pH未达标、温度过高或胶原浓度不足。

• 解决：验证pH（7.0–7.5）、确保低温操作（2–10°C）、核对浓度（3 mg/mL）。

• 问题2：细胞附着率低

• 原因：涂覆不均匀或胶原降解。

• 解决：优化涂覆厚度（推荐50–100 μg/cm²）、使用无菌操作工具。

• 问题3：凝胶机械性能不稳定

• 原因：胶原纯度不足或储存条件不当。

• 解决：验证批次纯度（SDS-PAGE检测）、确保2–10°C储存。

免责声明

1. 本文内容基于品牌公开资料及行业常规信息整理，仅个人观点，具体以品牌信息文档为准。

2. 本产品仅供科研用途，不可用于临床诊断或人体治疗。

3. 使用前需阅读并遵守材料安全数据表（MSDS）中的安全规范。

4. 实验结果可能因操作条件、细胞类型及批次差异而波动，建议进行预实验验证。

5. 产品性能数据基于实验室测试，实际应用中需根据具体需求调整方案。

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5005-100ML	PureCol®, Bovine Collagen, 3 mg/mL, 100 mL	100ml	advancedbiomatrix
5248-1KIT	Ruthenium, Visible Light Photocrosslinking Kit	1KIT	advancedbiomatrix
5153-1KIT	Rat Tail Collagen Solution, Acid Soluble with Neutralization Solution, 100 mg*(5153-100MG+5155-10ML)	1KIT	advancedbiomatrix
5007-20ml	Type I Collagen Solution, 3 mg/ml (Human)	20ml	advancedbiomatrix
5133-20ML	FibriCol®	20ml	advancedbiomatrix
5022-5MG	Human Collagen, Type IV	5MG	advancedbiomatrix
5015	PureCol®-S Type I Collagen Standard	20ml	advancedbiomatrix
5142-5EA	CytoSoft® 6-well Plate, Elastic Modulus 8 kPa	5EA	advancedbiomatrix
5144-5EA	CytoSoft® 6-well Plate, Elastic Modulus 32 kPa	5EA	advancedbiomatrix
5145-5EA	CytoSoft® 6-well Plate, Elastic Modulus 64 kPa	5EA	advancedbiomatrix
5165-5EA	CytoSoft® 6-well Plate, Elastic Modulus 0.2 kPa	5EA	advancedbiomatrix
5141-5EA	CytoSoft® 6-well Plate, Elastic Modulus 2kPa	5EA	advancedbiomatrix
5143-5EA	CytoSoft® 6-well Plate, Elastic Modulus 16 kPa	5EA	advancedbiomatrix
5190-7EA	CytoSoft® Discovery Kit	7EA	advancedbiomatrix
5079-50ml	Acetic Acid Solution, 20 mM, 50 mL	50ml	advancedbiomatrix
5140-5EA	CytoSoft® 6-well Plate, Elastic Modulus 0.5 kPa	5EA	advancedbiomatrix
5008	VitroCol® Lyophilized, 15 mg	15 mg	advancedbiomatrix
5021-10MG	Human Collagen Solution, Type III, 10 mg	10MG	advancedbiomatrix
5050-1MG	Fibronectin, Human, 1.0 mg	1MG	advancedbiomatrix
5185-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 2 kPa	1EA	advancedbiomatrix
5188-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 32 kPa	1EA	advancedbiomatrix
5201-1KIT	PhotoCol®-IRG	1kit	advancedbiomatrix
GS311F	HyStem® Thiol-Modified Hyaluronic Acid Kit 7.5 mL	7.5ml	advancedbiomatrix
5010-50ML	Type I Collagen Solution, 6 mg/ml (Bovine)	50mL	advancedbiomatrix
5026	TeloCol®-3 Type I Collagen Solution, 3 mg/ml (Bovine)	1kit	advancedbiomatrix
5056-20ML	Rat Tail Collagen Solution, Acid Soluble, 4 mg/ml	20ML	advancedbiomatrix
5225-1KIT	TeloCol®-6, Solution, Acid Soluble, 6 mg/ml + Neutralization Solution	1KIT	advancedbiomatrix
5226-20ML	TeloCol®-10	20ml	advancedbiomatrix
GS1006F	HyStem®-HP Thiol-Modified Hyaluronic Acid/Heparin 12.5 mL Kit (Experienced Users Only)	12.5ml	advancedbiomatrix
5016-5MG	Human Collagen, Type IV, Powder, Non-sterile, 5 mg	5MG	advancedbiomatrix
5020-5MG	PEPTITE-2000®, RGD Peptide, 5 mg	5MG	advancedbiomatrix
5051-0.1MG	Vitronectin, Human, 0.1 mg	0.1MG	advancedbiomatrix
5052-1MG	Tropoelastin, Human, Recombinant, Lyophilized, 1 mg	1MG	advancedbiomatrix
5073-5EA	6-well Plates, PureCol® Collagen Coated	5ea	advancedbiomatrix
5135-25EA	SpongeCol®, Collagen Sponges, Columnar Pore Architecture, 4 mm Diameter Discs	25ea	advancedbiomatrix
5162-1GM	Insoluble Collagen Type I Collagen, Insoluble Fibrous Powder (Bovine)	1GM	advancedbiomatrix
5183-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 0.2 kPa	1EA	advancedbiomatrix
5186-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 8 kPa	1EA	advancedbiomatrix
5189-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 64 kPa	1EA	advancedbiomatrix
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5255-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 0.2 kPa	1EA	advancedbiomatrix
5256-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 0.5 kPa	1EA	advancedbiomatrix
5257-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 2 kPa	1EA	advancedbiomatrix
5259-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 16 kPa	1EA	advancedbiomatrix
5261-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 64 kPa	1EA	advancedbiomatrix
5270-1KIT	PhotoCol®-LAP	1kit	advancedbiomatrix
5278-5ML	Lifeink® 200 Collagen Bioink, pH Neutral, 35 mg/ml	5ML	advancedbiomatrix
5316-10EA	CytoSoft®, T-25 Flasks, Elastic Modulus 0.2 kPa	10ea	advancedbiomatrix
5321-10EA	CytoSoft®, T-25 Flasks, Elastic Modulus 32 kPa	10ea	advancedbiomatrix
5323-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 0.2 kPa	6EA	advancedbiomatrix
5325-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 2 kPa	6EA	advancedbiomatrix
5328-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 32 kPa	6EA	advancedbiomatrix
5329-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 64 kPa	6ea	advancedbiomatrix
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GS231F-5EA	Gelin-S® Thiol-Modified Gelatin (5 units x 1 mL)	5EA	advancedbiomatrix
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5006-15MG	PureCol®, Bovine Collagen, Lyophilized, 15 mg	15MG	advancedbiomatrix
5007-100ML	VitroCol®, Human Collagen, Solution, 3 mg/mL	100ML	advancedbiomatrix
5010-D	Nutragen® Bovine Collagen	each	advancedbiomatrix
5019-10MG	Human Collagen, Type III, Powder, Non-sterile, 10 mg	10MG	advancedbiomatrix
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5135-5EA	SpongeCol®, Collagen Sponges, Columnar Pore Architecture, 21 mm Diameter Discs	5ea	advancedbiomatrix
5138-10GM	SphereCol®, Human Type I Collagen Coated Beads, Sterile, 10 grams	10GM	advancedbiomatrix
5154-20ML	Silk Fibroin Solution, 50 mg/ml	20ML	advancedbiomatrix
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5173-100MG	Poly-L-Lysine, Non-Sterile Powder, 100 mg	100MG	advancedbiomatrix
5174-100MG	Poly-D-Lysine, Non-Sterile Powder, 100 mg	100MG	advancedbiomatrix
5174-5MG	Poly-D-Lysine, Powder, 5 mg	5MG	advancedbiomatrix
5184-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 0.5 kPa	1EA	advancedbiomatrix
5187-1EA	CytoSoft® Imaging 24-well Plate, Elastic Modulus 16 kPa	1EA	advancedbiomatrix
5198-100MG	PhotoCol® Methacrylated Type I Collagen (Collagen Only)	100MG	advancedbiomatrix
5212-100MG	Methacrylated 100MGHyaluronic Acid (HAMA)	100MG	advancedbiomatrix
5220-1KIT	PhotoHA®-IRG Methacrylated Hyaluronic Acid with Irgacure Kit	1kit	advancedbiomatrix
5258-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 8 kPa	1EA	advancedbiomatrix
5260-1EA	CytoSoft® Imaging 96-well Plate, Elastic Modulus 32 kPa	1EA	advancedbiomatrix
5264-60UG	5-FAM Conjugate Collagen Hybridizing Peptide (Green)	60UG	advancedbiomatrix
5265-60UG	Biotin Conjugate Collagen Hybridizing Peptide (Biotin)	60UG	advancedbiomatrix
5267-5ML	Lifeink® 240 Collagen Bioink, Acidic pH, 35 mg/ml, 5 mL	5ML	advancedbiomatrix
5269-500mg	LAP	500mg	advancedbiomatrix
5276-60UG	CY3 Conjugate Collagen Hybridizing Peptide (Red)	60UG	advancedbiomatrix
5279	RatCol® High Concentration Rat Tail Type l Collagen, Intact Telo-peptide, (20 mM Acetic Acid)	ea	advancedbiomatrix
5310-200MG	PhotoAlginate®, Methacrylated Alginate, 200 mg	200MG	advancedbiomatrix
5315-5EA	Collagen Membrane Film Discs (23 mm diameter) - Pack of 5	5EA	advancedbiomatrix
5317-10EA	CytoSoft®, T-25 Flasks, Elastic Modulus 0.5 kPa	10ea	advancedbiomatrix
5324-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 0.5 kPa	6ea	advancedbiomatrix
5327-6EA	CytoSoft®, T-75 Flasks, Elastic Modulus 16 kPa	6EA	advancedbiomatrix
5339-1GM	PEGDA 6,000 MW bulk non-sterile powder, 1 gram	1GM	advancedbiomatrix