产品简介

       WPI公司最新上市的点针细胞电穿孔仪是一款简单、通用的细胞膜或细胞壁的穿透设备， 可用于将DNA、iRNA等遗传信息物质注入胚胎细胞以及植入前期的哺乳动物胚胎中，可在不撕裂或破坏细胞膜的情况下，实现小创伤、不留痕迹 、精确的膜穿透。

产品特征

● 触摸屏显示：电阻式触摸屏，戴手套也可方便使用; 仪器操作模式：脚踏开关控制注射或触摸屏手动注射控制 ;
● 直观的用户界面;占用空间小;
● 参数设置：通过触摸屏或旋钮调节频率和电压; 电穿孔程序：四种用户可编程程序;
● 警示音：可调音频连续音表示有效探头; 计数显示：注射计数器指示注射总次数;
● 通用性：电极支架与大多数常见操纵器兼容; 高存活率：小鼠胚胎双细胞期注射后约80-85% 存活率;

装在倒置显微镜下的点针细胞电穿孔仪。可以兼容WPI公司SYS-PV830，艾本德公司的FemtoJet注射系统。

这是一个典型的微注射装置，使用MICRO-ePORE™、一个INV-101倒置显微镜和一个PV830气动皮升操作泵。

产品用途

● 微量注射到卵母细胞和植入前期的哺乳动物胚胎, 包括将CRISPR-Cas9试剂注射到双细胞胚胎的细胞质中;
● 原核啮齿动物受精卵微量注射;
● 斑马鱼中的基因沉默;
 

江西某高校斑马鱼中心使用第二代原型机在斑马鱼尾鳍注入Morpholinos。

参考文献

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