13530144996
Collagenase, Type 2 (CLS-2)2型胶原酶
货号:LS004176
CAS：9001.12.1
品名:Collagenase, Type 2 (CLS-2)2型胶原酶
品牌:Worthington-biochem
规格:1gm
价格:请询价
货期:现货
关键词:
Worthington胶原酶Type2、原代细胞分离、心脏组织解离、骨骼细胞培养、生物制药酶制剂、溶组织梭菌来源、高纯度胶原酶、细胞治疗原料、实验室组织消化、冻干酶复溶、GMP认证酶、类器官培养试剂、肿瘤样本处理、心肌细胞分离、肝细胞消化、3D生物打印、科研级酶制剂、无动物源胶原酶、细胞活性保护、胶原酶活性检测
产品详情介绍
一、产品基础信息
• 货号：LS004176
• 英文名：Collagenase, Type 2 (CLS-2)
• 中文名：2型胶原酶
• 品牌：Worthington-biochem
• 规格：1克（冻干粉末）
• CAS号：9001-12-1
• 来源：溶组织梭菌（Clostridium histolyticum）发酵产物
• 活性单位：≥125 U/mg（基于改良Mandl法测定）
• 保存条件：2-8°C干燥避光保存，有效期2年
二、核心作用原理
2型胶原酶是一种复合型酶制剂，其核心功能是通过特异性水解胶原蛋白的三螺旋结构，瓦解细胞外基质（ECM），实现组织解离。其作用机制包括：
1. 靶向胶原切割：优先识别并切割天然胶原中Pro-X-Gly-Pro序列的Y-Gly键（Y为中性和芳香族氨基酸），破坏胶原纤维的稳定性。
2. 协同蛋白酶活性：除胶原酶活性外，含高水平的梭菌蛋白酶（Clostripain），可辅助降解其他细胞外基质蛋白（如弹性蛋白、纤维连接蛋白）及部分膜蛋白。
3. 温和解离特性：相较于胰蛋白酶等强效蛋白酶，其对细胞膜表面受体的损伤更小，适用于需保持细胞活性的实验。
三、解决的关键实验问题
1. 组织解离效率低：针对心脏、骨骼、肝脏等富含胶原的组织，传统胰酶或单一酶解离效果差，CLS-2通过高胶原酶活性显著提升解离效率。
2. 细胞损伤与活性损失：高纯度设计减少非特异性蛋白水解，降低细胞膜蛋白破坏，保障原代细胞存活率（文献报道活性细胞得率可达85%以上）。
3. 批次间差异：Worthington严格的质控体系确保每批次酶活性稳定，避免因酶活性波动导致的实验可重复性问题。
4. 复杂组织处理：适用于心脏、甲状腺、唾液腺等坚韧组织的分离，尤其适合需要保留细胞间连接的研究场景。
四、产品核心优势
1. 高纯度与活性：
• 采用溶组织梭菌发酵工艺，避免动物源成分污染，符合GMP及生物制药标准。
• 活性单位定义严格（37°C、pH7.5下5小时水解胶原释放1μmol亮氨酸为1单位），确保实验数据可靠性。
2. 应用广泛性：
• 科研领域：原代心肌细胞、肝细胞、骨细胞分离；肿瘤组织消化；3D类器官培养。
• 工业领域：疫苗生产、生物制品纯化、细胞治疗原料制备。
3. 技术适配性：
• 可与弹性蛋白酶、透明质酸酶等联用，定制化优化解离方案。
• 支持冻干复溶，长期保存不影响活性。
4. 安全性保障：
• 通过ISO9001认证，生产流程全程可追溯。
• 无动物源性成分（部分批次需特殊说明），降低外源病原体风险。
五、目标客户群体
1. 科研机构：高校、研究所的细胞生物学、组织工程实验室。
2. 生物制药企业：疫苗研发、CAR-T细胞治疗、干细胞制备部门。
3. 临床诊断实验室：病理科组织样本处理、循环肿瘤细胞分离。
4. CRO/CDMO公司：需标准化酶解工艺的合同研发与生产服务。
六、订货信息
• 购买渠道：深圳亚舟生物科技有限公司
• 规格选项：1克、5克、10克（支持定制化分装）。
• 运输方式：冷链运输（干冰包装），确保活性稳定。
七、常见问题与解答
1. Q：CLS-2与Type 1胶原酶有何区别？
• A：Type 1胶原酶含更均衡的蛋白酶谱（如酪蛋白酶、胰蛋白酶活性），适用于上皮、脂肪组织；Type 2侧重高梭菌蛋白酶活性，更适合心脏、骨骼等坚韧组织。
2. Q：如何判断酶活性是否达标？
• A：建议通过胶原水解实验验证。
3. Q：是否支持低温解离？
• A：推荐37°C孵育以保持酶活性，低温（4°C）下反应速率显著降低，可能需延长消化时间。
4. Q：储存过程中出现结块是否影响使用？
• A：轻微结块可研磨后使用，若严重吸潮需联系供应商更换。
5. Q：是否适用于类器官培养？
• A：是的，其温和特性可减少类器官结构损伤，推荐与基质胶联合使用。
关键词：Worthington胶原酶Type2、原代细胞分离、心脏组织解离、骨骼细胞培养、生物制药酶制剂、溶组织梭菌来源、高纯度胶原酶、细胞治疗原料、实验室组织消化、冻干酶复溶、GMP认证酶、类器官培养试剂、肿瘤样本处理、心肌细胞分离、肝细胞消化、3D生物打印、科研级酶制剂、无动物源胶原酶、细胞活性保护、胶原酶活性检测
（注：本文内容基于品牌公开资料及行业常规信息整理，具体以品牌信息文档为准。）
⚡Worthington热卖产品
|
货号 |
品名 |
规格 |
品牌 |
|
LS004176,CAS：9001.12.1, |
Collagenase, Type 2 (CLS-2)2型胶原酶 |
1gm |
Worthington-biochem |
|
LS004196,原始货号：LS004200,CAS：9001.12.1 |
Collagenase Type 1 |
1gm |
Worthington-biochem |
|
LS004188,CAS：9001.12.1, |
Collagenase, Type 4 |
1gm |
Worthington-biochem |
|
LS004194,CAS：9001.12.1, |
Collagenase Type 1 (CLS-1) |
100mg |
Worthington-biochem |
|
LS004174,CAS：9001.12.1, |
Collagenase, Type 2 |
100mg |
Worthington-biochem |
|
LS004186,CAS：9001.12.1, |
Collagenase, Type 4 |
100mg |
Worthington-biochem |
|
LS004182,CAS：9001.12.1, |
Collagese, Type 3 |
1gm |
Worthington-biochem |
|
ls003126,CAS：9001.73.4, |
Papain, Suspension |
100mg |
Worthington-biochem |
|
LK003150,原始货号：LK003153, |
Papain Dissociation System |
1bx |
Worthington-biochem |
|
LS003119,CAS：9001.73.4, |
Papain, Lyophilized |
100mg |
Worthington-biochem |
|
LS004180,CAS：9001.12.1, |
Collagenase, Type 3 |
100mg |
Worthington-biochem |
|
LS003740,CAS：9002.07.7, |
Trypsin, TPCK Treated |
100mg |
Worthington-biochem |
|
LS002139,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS002292,CAS：39445.21.1, |
Elastase Lyophilized |
100mg |
Worthington-biochem |
|
LK003178,CAS：9001.73.4, |
PDS Kit Papain Vial |
5vi |
Worthington-biochem |
|
LS002279,CAS：39445.21.1, |
Elastase, Suspension (ES) |
100mg |
Worthington-biochem |
|
LS004177,CAS：9001.12.1, |
Collagese, Type 2 |
5gm |
Worthington-biochem |
|
LS002006,CAS：9003.98.9, |
Deoxyribonuclease I |
20mg |
Worthington-biochem |
|
LS002007,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS004189,CAS：9001.12.1, |
Collagenase, Type 4 |
5 gm |
Worthington-biochem |
|
LK003176,原始货号：LK003178,CAS：9001.73.4 |
PDS Kit Papain Vial (PAP2) |
1 vi |
Worthington-biochem |
|
LS002140,CAS：9003.98.9, |
Deoxyribonuclease I |
1 gm |
Worthington-biochem |
|
LS003926,CAS：9025.82.5, |
Phosphodiesterase I |
100un |
Worthington-biochem |
|
LK003182,CAS：9035.81.8/9048.46.8, |
PDS Kit Inhibitor Vial (OI-BSA) |
1 vi |
Worthington-biochem |
|
LS05006,CAS：9068.38.6, |
Reverse Transcriptase, Recombinant, HIV |
5x200 un |
Worthington-biochem |
|
LS002594,CAS：3726.33.3, |
Hyaluronidase |
50ku |
Worthington-biochem |
|
LS004106,CAS：9001.12.1/42613.33.2, |
STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free |
50mg |
Worthington-biochem |
|
LS02100,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
10mg |
Worthington-biochem |
|
LS002290,CAS：39445.21.1, |
Elastase, Lyophilized |
25mg |
Worthington-biochem |
|
LK003170,原始货号：LK003172,CAS：9003.98.9 |
DNase Vial (D2) (D2 |
1 vi |
Worthington-biochem |
|
LS003570,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
100mg |
Worthington-biochem |
|
LS003608,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
1mg |
Worthington-biochem |
|
LS002274,CAS：39445.21.1, |
Elastase, Suspension |
25mg |
Worthington-biochem |
|
LS003127,CAS：9001.73.4, |
Papain, Suspension |
1 gm |
Worthington-biochem |
|
LS003086,CAS：9035.81.8, |
Trypsin Inhbitor, Ovomucoid |
2 gm |
Worthington-biochem |
|
LS003118,CAS：9001.73.4, |
Papain, Lyophilized |
25mg |
Worthington-biochem |
|
LS004212,CAS：9001.12.1, |
Collagenase, Type 4, Filtered Store at 2-8°C. |
5x50mg |
Worthington-biochem |
|
LS004200,CAS：9001.12.1, |
Collagenase, Type 1 |
Bulk |
Worthington-biochem |
|
LS005305,CAS：9025.24.5, |
Carboxypeptidase B |
5mg |
Worthington-biochem |
|
LS004179,CAS：9001.12.1, |
Collagese, Type 2 |
Bulk |
Worthington-biochem |
|
LS004197,CAS：9001.12.1, |
Collagenase Type 1 |
5 gm |
Worthington-biochem |
|
LS003120,CAS：9001.73.4, |
Papain, Lyophilized |
1 gm |
Worthington-biochem |
|
LS004191,CAS：9001.12.1, |
Collagenase, Type 4 |
500g |
Worthington-biochem |
|
LS006344,CAS：9003.98.9, |
Deoxyribonuclease I, RNase & Protease Free, Solution |
500 un |
Worthington-biochem |
|
LS004798,CAS：9013.53.0, |
Nuclease, Micrococcal |
45 ku |
Worthington-biochem |
|
LS02104,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
50mg |
Worthington-biochem |
|
LS003124,CAS：9001.73.4, |
Papain, Suspension |
25mg |
Worthington-biochem |
|
LS003571,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
1 gm |
Worthington-biochem |
|
LS004520,CAS：9028.79.9, |
Galactose Oxidase |
150 un |
Worthington-biochem |
|
LS004522,CAS：9028.79.9, |
Galactose Oxidase |
450 un |
Worthington-biochem |
|
LS001070,CAS：9031-72-5, |
Alcohol Dehydrogese, Lyophilized |
1gm |
Worthington-biochem |
|
LS002138,CAS：9003.98.9, |
Deoxyribonuclease I |
25mg |
Worthington-biochem |
|
LS002592,CAS：3726.33.3, |
Hyaluronidase |
300ku |
Worthington-biochem |
|
LS003793,CAS：9002.10.2, |
Polyphenol Oxidase (Tyrosinase) |
500 ku |
Worthington-biochem |
|
LS004524,CAS：9028.79.9, |
Galactose Oxidase |
1 ku |
Worthington-biochem |
|
LS003605,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
5mg |
Worthington-biochem |
|
LK003163,, |
Papain Dissociation System, Without EBSS |
3 bx |
Worthington-biochem |
|
LS005275,CAS：9001.12.1, |
Collagenase, Purified |
4ku |
Worthington-biochem |
|
LS003741,CAS：9002.07.7, |
Trypsin, TPCK Treated (TRTPCK) |
500mg |
Worthington-biochem |
|
LS05003,CAS：9068.38.6, |
everse Transcriptase Recombinant HIV |
200 un |
Worthington-biochem |
|
LS02111,CAS：42613.33.2, |
Neutral Protease, Partially Purified |
5gm |
Worthington-biochem |
|
LS003587,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
1 gm |
Worthington-biochem |
|
LS006323（500ku）,, |
Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade |
500ku |
Worthington-biochem |
|
LS002933,CAS：12650.88.3, |
Lysozyme, Purified, Salt Free |
5 gm |
Worthington-biochem |
|
LS006123,CAS：9001.78.9, |
Phosphatase, Alkaline, Purified |
25mg |
Worthington-biochem |
|
LS006363,CAS：39445.21.1, |
Elastase, Purified |
5mg |
Worthington-biochem |
|
LS005282,CAS：9001.12.1, |
Collagenase, Type 5 |
1gm |
Worthington-biochem |
|
LS02122,CAS：9002.07.7, |
Trypsin, Modified, SequENZ® Sequencing Grade |
4x100ug |
Worthington-biochem |
|
LS003744,CAS：9002.07.7, |
Trypsin, TPCK Treated |
1 gm |
Worthington-biochem |
|
LS005477,CAS：3726.33.3, |
Hyaluronidase, Purified |
5ku |
Worthington-biochem |
|
LS003589,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
10gm |
Worthington-biochem |
|
LS001141,CAS：9001.77.8, |
Phosphatase, Acid |
1gm |
Worthington-biochem |
|
LS004185,CAS：9001.12.1, |
Collagenase, Type 3 |
500g |
Worthington-biochem |
|
LS003602,CAS：9068.54.6, |
Phosphodiesterase II |
25un |
Worthington-biochem |
|
LS01492,CAS：9026.12.4, |
Ribonuclease T1, Chromatographically Purif., Lyophilized |
2500ku |
Worthington-biochem |
|
LS001656,CAS：9007-34-5, |
Collagen |
10 gm |
Worthington-biochem |
|
LS002106,CAS：9007.49.2, |
Deoxyribonucleic Acid, Calf Thymus |
1 gm |
Worthington-biochem |
|
LS001334,CAS：9004.07.3, |
Chymotrypsin, Alpha, 1X |
10 gm |
Worthington-biochem |
|
LS004780,CAS：9001.67.6, |
Neuraminidase |
10mg |
Worthington-biochem |
|
LS003887,CAS：9002.13.5, |
Urease |
10 gm |
Worthington-biochem |
|
LK003153,, |
Papain Dissociation System |
3bx |
Worthington-biochem |
|
LS001263,CAS：9001.03.0, |
Carbonic Anhydrase |
250mg |
Worthington-biochem |
|
LS004154,CAS：9001.12.1, |
Collagenase, Type A, Animal Free |
1gm |
Worthington-biochem |
|
LS004081,CAS：9001.78.9, |
Phosphatase, Alkaline |
10mg |
Worthington-biochem |
|
LK003172,CAS：9003.98.9, |
DNase Vial (D2) |
5 vi |
Worthington-biochem |
|
LS002764,CAS：9000.89.9, |
Amino Acid Oxidase, L- |
5mg |
Worthington-biochem |
|
LS003981,CAS：9001.50.7, |
Glucose-6-Phosphate Dehydrogenase, Lyophilized |
1 ku |
Worthington-biochem |
|
LS004326-100ku,, |
Diaphorase |
100 ku |
Worthington-biochem |
|
LK003188,, |
PDS Kit, EBSS Via |
1vi |
Worthington-biochem |
|
LS004759,CAS：9001.67.6, |
Neuraminidase, Purified |
5 un |
Worthington-biochem |
|
LS005304,CAS：9025.24.5, |
Carboxypeptidase B |
50mg |
Worthington-biochem |
|
LS006355,CAS：9003.98.9, |
Deoxyribonuclease I, Recombinant, Solution |
5x2 ku |
Worthington-biochem |
|
LS006365,CAS：39445.21.1, |
Elastase, Purified |
20mg |
Worthington-biochem |
|
LK003300,, |
Neonatal Cardiomyocyte Isolation System |
1 kt |
Worthington-biochem |
|
LS02123,CAS：9002.07.7, |
Trypsin, Modified, Sequencing Grade |
1mg |
Worthington-biochem |
|
LS002976,CAS：84195.52.8, |
Mucin |
500mg |
Worthington-biochem |
|
LS01502,CAS：37278.25.4, |
Ribonuclease T2, Recombinant |
250ku |
Worthington-biochem |
|
LS002512,, |
Hexokinase, Lyophilized |
10 ku |
Worthington-biochem |
|
LS004206,CAS：9001.12.1, |
Collagenase, Type 3, Filtered |
50mg |
Worthington-biochem |
|
LS006308,CAS：9003.98.9, |
Amino Acid Oxidase, D- |
25mg |
Worthington-biochem |