13530144996
Collagenase, Type 3（胶原蛋白酶）
货号:LS004180
品名:Collagenase, Type 3（胶原蛋白酶）
品牌:Worthington-biochem
规格:100mg
价格:请询价
货期:现货
关键词:
Worthington胶原蛋白酶Type3、原代细胞分离酶、细胞培养胶原蛋白酶、Type3胶原酶说明书、乳腺细胞分离酶、软组织解离酶、冻干胶原蛋白酶、低蛋白酶活性酶、流式细胞分选酶、单细胞测序酶、生物制药级胶原酶、组织消化酶LS004180、Clostridium histolyticum来源酶、细胞治疗研究试剂、酶解优化方案、高纯度胶原酶、实验室常用酶、细胞分离技术、科研试剂货号LS004180、Worthington生物酶产品
产品详情介绍
产品描述
货号：LS004180
英文名：Collagenase, Type 3, Filtered
中文名：胶原蛋白酶，Type 3（过滤型）
品牌：Worthington-biochem
规格：100mg
CAS号：9001.12.1
来源：由Clostridium histolyticum发酵纯化，并经0.22微米滤膜过滤，冻干保存。
应用领域：专用于哺乳动物组织的原代细胞分离，适用于细胞培养、流式细胞分选、单细胞测序及生物制药研究。
产品特点
1. 高纯度与活性：采用先进纯化技术，胶原蛋白酶活性≥100 U/mg，次级蛋白酶活性极低（≤50 U/mg），减少对目标细胞的损伤。
2. 精准应用：Type 3类型针对低蛋白酶需求的组织，避免膜蛋白过度降解，保障细胞功能完整性。
3. 稳定性强：冻干粉形式可在2-8°C长期保存（1年），复溶后建议分装冷冻，避免反复冻融。
4. 兼容性广：支持与中性蛋白酶、透明质酸酶等联用，优化不同组织的解离效率。
储存条件
• 短期储存：2-8°C避光保存，避免潮湿。
• 长期储存：-20°C冻存（复溶后需分装）。
• 保质期：自生产日起12个月（未开封）。
工作原理
胶原蛋白酶通过水解胶原蛋白的三螺旋结构，破坏细胞间基质，释放目标细胞。Type 3的酶谱设计使其在低蛋白酶活性下高效解离软组织，同时保留细胞表面受体和膜蛋白功能，适用于对酶解损伤敏感的实验。
解决实验中哪些问题
1. 组织分离效率低：针对乳腺、脂肪等富含胶原的组织，显著提升细胞得率。
2. 细胞损伤大：低次级蛋白酶活性减少细胞膜损伤，适用于流式分选和类器官培养。
3. 消化时间不可控：与中性蛋白酶联用可缩短消化周期，提高实验可重复性。
4. 成本优化：高纯度减少酶用量，降低实验成本。
使用方法
1. 解冻与复溶：
• 4°C缓慢解冻冻干粉，避免剧烈震荡。
• 加入适量预冷缓冲液，轻轻溶解。
2. 酶活性验证：
• 取1 mg酶粉溶于1 mL缓冲液，37°C孵育1小时，检测胶原降解能力。
3. 组织消化流程：
• 组织剪碎至<1 mm³，加入含胶原蛋白酶的缓冲液。
• 37°C振荡孵育，定期取样观察解离程度。
• 加入含10%胎牛血清的培养基终止反应，离心收集细胞。
4. 中和处理：
• 若使用含EDTA的缓冲液，需用含Ca²⁺/Mg²⁺的培养基中和。
常见问题与解决方案
• 问题1：酶活性低于预期
原因：储存温度过高或反复冻融。
方案：验证活性后分装冷冻；复溶时使用无核酸酶水。
• 问题2：细胞碎片残留多
原因：组织未充分剪碎或酶浓度不足。
方案：预冷PBS冲洗组织，增加酶浓度至2 mg/mL。
• 问题3：消化时间差异大
原因：组织类型或密度不同。
方案：预实验确定最佳时间。
• 问题4：细胞存活率低
原因：血清终止不及时或温度过高。
方案：消化后立即加入含血清培养基，全程冰上操作。
免责声明
1. 本产品为科研试剂，不可用于临床诊断或治疗。
2. 实验结果可能因操作环境、样本差异等因素波动，建议参考Worthington官方技术网页优化方案。
3. 产品不包含任何生物安全认证，使用者需自行评估实验风险。
4. 质量控制标准以出厂检测报告为准，运输过程可能导致活性轻微波动。
5. 本文内容基于品牌公开资料及行业常规信息整理，仅个人观点，具体以品牌信息文档为准。
关键词：Worthington胶原蛋白酶Type3、原代细胞分离酶、细胞培养胶原蛋白酶、Type3胶原酶说明书、乳腺细胞分离酶、软组织解离酶、冻干胶原蛋白酶、低蛋白酶活性酶、流式细胞分选酶、单细胞测序酶、生物制药级胶原酶、组织消化酶LS004180、Clostridium histolyticum来源酶、细胞治疗研究试剂、酶解优化方案、高纯度胶原酶、实验室常用酶、细胞分离技术、科研试剂货号LS004180、Worthington生物酶产品
⚡Worthington热卖产品
|
货号 |
品名 |
规格 |
品牌 |
|
LS004176,CAS：9001.12.1, |
Collagenase, Type 2 (CLS-2)2型胶原酶 |
1gm |
Worthington-biochem |
|
LS004196,原始货号：LS004200,CAS：9001.12.1 |
Collagenase Type 1 |
1gm |
Worthington-biochem |
|
LS004188,CAS：9001.12.1, |
Collagenase, Type 4 |
1gm |
Worthington-biochem |
|
LS004194,CAS：9001.12.1, |
Collagenase Type 1 (CLS-1) |
100mg |
Worthington-biochem |
|
LS004174,CAS：9001.12.1, |
Collagenase, Type 2 |
100mg |
Worthington-biochem |
|
LS004186,CAS：9001.12.1, |
Collagenase, Type 4 |
100mg |
Worthington-biochem |
|
LS004182,CAS：9001.12.1, |
Collagese, Type 3 |
1gm |
Worthington-biochem |
|
ls003126,CAS：9001.73.4, |
Papain, Suspension |
100mg |
Worthington-biochem |
|
LK003150,原始货号：LK003153, |
Papain Dissociation System |
1bx |
Worthington-biochem |
|
LS003119,CAS：9001.73.4, |
Papain, Lyophilized |
100mg |
Worthington-biochem |
|
LS004180,CAS：9001.12.1, |
Collagenase, Type 3 |
100mg |
Worthington-biochem |
|
LS003740,CAS：9002.07.7, |
Trypsin, TPCK Treated |
100mg |
Worthington-biochem |
|
LS002139,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS002292,CAS：39445.21.1, |
Elastase Lyophilized |
100mg |
Worthington-biochem |
|
LK003178,CAS：9001.73.4, |
PDS Kit Papain Vial |
5vi |
Worthington-biochem |
|
LS002279,CAS：39445.21.1, |
Elastase, Suspension (ES) |
100mg |
Worthington-biochem |
|
LS004177,CAS：9001.12.1, |
Collagese, Type 2 |
5gm |
Worthington-biochem |
|
LS002006,CAS：9003.98.9, |
Deoxyribonuclease I |
20mg |
Worthington-biochem |
|
LS002007,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS004189,CAS：9001.12.1, |
Collagenase, Type 4 |
5 gm |
Worthington-biochem |
|
LK003176,原始货号：LK003178,CAS：9001.73.4 |
PDS Kit Papain Vial (PAP2) |
1 vi |
Worthington-biochem |
|
LS002140,CAS：9003.98.9, |
Deoxyribonuclease I |
1 gm |
Worthington-biochem |
|
LS003926,CAS：9025.82.5, |
Phosphodiesterase I |
100un |
Worthington-biochem |
|
LK003182,CAS：9035.81.8/9048.46.8, |
PDS Kit Inhibitor Vial (OI-BSA) |
1 vi |
Worthington-biochem |
|
LS05006,CAS：9068.38.6, |
Reverse Transcriptase, Recombinant, HIV |
5x200 un |
Worthington-biochem |
|
LS002594,CAS：3726.33.3, |
Hyaluronidase |
50ku |
Worthington-biochem |
|
LS004106,CAS：9001.12.1/42613.33.2, |
STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free |
50mg |
Worthington-biochem |
|
LS02100,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
10mg |
Worthington-biochem |
|
LS002290,CAS：39445.21.1, |
Elastase, Lyophilized |
25mg |
Worthington-biochem |
|
LK003170,原始货号：LK003172,CAS：9003.98.9 |
DNase Vial (D2) (D2 |
1 vi |
Worthington-biochem |
|
LS003570,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
100mg |
Worthington-biochem |
|
LS003608,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
1mg |
Worthington-biochem |
|
LS002274,CAS：39445.21.1, |
Elastase, Suspension |
25mg |
Worthington-biochem |
|
LS003127,CAS：9001.73.4, |
Papain, Suspension |
1 gm |
Worthington-biochem |
|
LS003086,CAS：9035.81.8, |
Trypsin Inhbitor, Ovomucoid |
2 gm |
Worthington-biochem |
|
LS003118,CAS：9001.73.4, |
Papain, Lyophilized |
25mg |
Worthington-biochem |
|
LS004212,CAS：9001.12.1, |
Collagenase, Type 4, Filtered Store at 2-8°C. |
5x50mg |
Worthington-biochem |
|
LS004200,CAS：9001.12.1, |
Collagenase, Type 1 |
Bulk |
Worthington-biochem |
|
LS005305,CAS：9025.24.5, |
Carboxypeptidase B |
5mg |
Worthington-biochem |
|
LS004179,CAS：9001.12.1, |
Collagese, Type 2 |
Bulk |
Worthington-biochem |
|
LS004197,CAS：9001.12.1, |
Collagenase Type 1 |
5 gm |
Worthington-biochem |
|
LS003120,CAS：9001.73.4, |
Papain, Lyophilized |
1 gm |
Worthington-biochem |
|
LS004191,CAS：9001.12.1, |
Collagenase, Type 4 |
500g |
Worthington-biochem |
|
LS006344,CAS：9003.98.9, |
Deoxyribonuclease I, RNase & Protease Free, Solution |
500 un |
Worthington-biochem |
|
LS004798,CAS：9013.53.0, |
Nuclease, Micrococcal |
45 ku |
Worthington-biochem |
|
LS02104,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
50mg |
Worthington-biochem |
|
LS003124,CAS：9001.73.4, |
Papain, Suspension |
25mg |
Worthington-biochem |
|
LS003571,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
1 gm |
Worthington-biochem |
|
LS004520,CAS：9028.79.9, |
Galactose Oxidase |
150 un |
Worthington-biochem |
|
LS004522,CAS：9028.79.9, |
Galactose Oxidase |
450 un |
Worthington-biochem |
|
LS001070,CAS：9031-72-5, |
Alcohol Dehydrogese, Lyophilized |
1gm |
Worthington-biochem |
|
LS002138,CAS：9003.98.9, |
Deoxyribonuclease I |
25mg |
Worthington-biochem |
|
LS002592,CAS：3726.33.3, |
Hyaluronidase |
300ku |
Worthington-biochem |
|
LS003793,CAS：9002.10.2, |
Polyphenol Oxidase (Tyrosinase) |
500 ku |
Worthington-biochem |
|
LS004524,CAS：9028.79.9, |
Galactose Oxidase |
1 ku |
Worthington-biochem |
|
LS003605,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
5mg |
Worthington-biochem |
|
LK003163,, |
Papain Dissociation System, Without EBSS |
3 bx |
Worthington-biochem |
|
LS005275,CAS：9001.12.1, |
Collagenase, Purified |
4ku |
Worthington-biochem |
|
LS003741,CAS：9002.07.7, |
Trypsin, TPCK Treated (TRTPCK) |
500mg |
Worthington-biochem |
|
LS05003,CAS：9068.38.6, |
everse Transcriptase Recombinant HIV |
200 un |
Worthington-biochem |
|
LS02111,CAS：42613.33.2, |
Neutral Protease, Partially Purified |
5gm |
Worthington-biochem |
|
LS003587,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
1 gm |
Worthington-biochem |
|
LS006323（500ku）,, |
Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade |
500ku |
Worthington-biochem |
|
LS002933,CAS：12650.88.3, |
Lysozyme, Purified, Salt Free |
5 gm |
Worthington-biochem |
|
LS006123,CAS：9001.78.9, |
Phosphatase, Alkaline, Purified |
25mg |
Worthington-biochem |
|
LS006363,CAS：39445.21.1, |
Elastase, Purified |
5mg |
Worthington-biochem |
|
LS005282,CAS：9001.12.1, |
Collagenase, Type 5 |
1gm |
Worthington-biochem |
|
LS02122,CAS：9002.07.7, |
Trypsin, Modified, SequENZ® Sequencing Grade |
4x100ug |
Worthington-biochem |
|
LS003744,CAS：9002.07.7, |
Trypsin, TPCK Treated |
1 gm |
Worthington-biochem |
|
LS005477,CAS：3726.33.3, |
Hyaluronidase, Purified |
5ku |
Worthington-biochem |
|
LS003589,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
10gm |
Worthington-biochem |
|
LS001141,CAS：9001.77.8, |
Phosphatase, Acid |
1gm |
Worthington-biochem |
|
LS004185,CAS：9001.12.1, |
Collagenase, Type 3 |
500g |
Worthington-biochem |
|
LS003602,CAS：9068.54.6, |
Phosphodiesterase II |
25un |
Worthington-biochem |
|
LS01492,CAS：9026.12.4, |
Ribonuclease T1, Chromatographically Purif., Lyophilized |
2500ku |
Worthington-biochem |
|
LS001656,CAS：9007-34-5, |
Collagen |
10 gm |
Worthington-biochem |
|
LS002106,CAS：9007.49.2, |
Deoxyribonucleic Acid, Calf Thymus |
1 gm |
Worthington-biochem |
|
LS001334,CAS：9004.07.3, |
Chymotrypsin, Alpha, 1X |
10 gm |
Worthington-biochem |
|
LS004780,CAS：9001.67.6, |
Neuraminidase |
10mg |
Worthington-biochem |
|
LS003887,CAS：9002.13.5, |
Urease |
10 gm |
Worthington-biochem |
|
LK003153,, |
Papain Dissociation System |
3bx |
Worthington-biochem |
|
LS001263,CAS：9001.03.0, |
Carbonic Anhydrase |
250mg |
Worthington-biochem |
|
LS004154,CAS：9001.12.1, |
Collagenase, Type A, Animal Free |
1gm |
Worthington-biochem |
|
LS004081,CAS：9001.78.9, |
Phosphatase, Alkaline |
10mg |
Worthington-biochem |
|
LK003172,CAS：9003.98.9, |
DNase Vial (D2) |
5 vi |
Worthington-biochem |
|
LS002764,CAS：9000.89.9, |
Amino Acid Oxidase, L- |
5mg |
Worthington-biochem |
|
LS003981,CAS：9001.50.7, |
Glucose-6-Phosphate Dehydrogenase, Lyophilized |
1 ku |
Worthington-biochem |
|
LS004326-100ku,, |
Diaphorase |
100 ku |
Worthington-biochem |
|
LK003188,, |
PDS Kit, EBSS Via |
1vi |
Worthington-biochem |
|
LS004759,CAS：9001.67.6, |
Neuraminidase, Purified |
5 un |
Worthington-biochem |
|
LS005304,CAS：9025.24.5, |
Carboxypeptidase B |
50mg |
Worthington-biochem |
|
LS006355,CAS：9003.98.9, |
Deoxyribonuclease I, Recombinant, Solution |
5x2 ku |
Worthington-biochem |
|
LS006365,CAS：39445.21.1, |
Elastase, Purified |
20mg |
Worthington-biochem |
|
LK003300,, |
Neonatal Cardiomyocyte Isolation System |
1 kt |
Worthington-biochem |
|
LS02123,CAS：9002.07.7, |
Trypsin, Modified, Sequencing Grade |
1mg |
Worthington-biochem |
|
LS002976,CAS：84195.52.8, |
Mucin |
500mg |
Worthington-biochem |
|
LS01502,CAS：37278.25.4, |
Ribonuclease T2, Recombinant |
250ku |
Worthington-biochem |
|
LS002512,, |
Hexokinase, Lyophilized |
10 ku |
Worthington-biochem |
|
LS004206,CAS：9001.12.1, |
Collagenase, Type 3, Filtered |
50mg |
Worthington-biochem |
|
LS006308,CAS：9003.98.9, |
Amino Acid Oxidase, D- |
25mg |
Worthington-biochem |