13530144996
Collagese, Type 3(胶原蛋白酶，Type 3型)
货号:LS004182
品名:Collagese, Type 3(胶原蛋白酶，Type 3型)
品牌:Worthington-biochem
规格:1gm
价格:请询价
货期:现货
关键词:
Worthington胶原蛋白酶Type3、原代细胞分离酶、冻干粉酶制剂、Type3胶原酶、细胞解离酶、肿瘤组织消化、器官灌注实验、低损伤细胞分离、高纯度胶原酶、科研级酶制剂、胶原酶Type3应用、细胞培养酶、冻干酶保存、酶活性稳定、多酶协同解离、HBSS缓冲液配方、细胞活性保护、实验室常用酶、Worthington生物酶、胶原酶技术参数
产品详情介绍
产品描述
货号：LS004182
英文名：Collagenase, Type 3, Filtered
中文名：胶原蛋白酶，Type 3型（过滤型）
品牌：Worthington-Biochem
规格：1克（冻干粉）
CAS号：9001-12-1
来源：溶组织梭菌发酵提取，经0.22 μm滤膜过滤及冻干工艺处理。
应用领域：生命科学研究、原代细胞分离、肿瘤组织解离、器官灌注实验等。
产品特点
1. 高纯度与活性：采用先进纯化技术，确保酶活性稳定，适用于对蛋白酶污染敏感的实验。
2. 温和消化：特异性切割胶原蛋白三螺旋结构，减少对细胞膜和其他细胞外基质的损伤，适用于乳腺、脂肪、肝脏等组织的原代细胞分离。
3. 多酶协同：除胶原酶外，含少量梭菌蛋白酶和中性蛋白酶，可协同降解结缔组织中的其他成分，提升组织解离效率。
4. 冻干工艺：冻干粉形态便于长期保存（2-8°C可稳定12个月），复溶后活性保持率高。
5. 低内毒素：生产过程严格把控，内毒素含量<0.1 EU/mg，适用于细胞培养等敏感实验。
储存条件
• 未开封：2-8°C避光干燥保存，避免冷冻。
• 开封后：分装为单次用量，-20°C短期保存（建议1个月内用完），避免反复冻融。
• 复溶后溶液：分装后-80°C冻存，避免反复解冻。
工作原理
胶原酶通过特异性水解胶原蛋白的Pro-X-Gly-Pro序列，破坏其三螺旋结构，从而降解细胞间质中的胶原纤维。Type 3型胶原酶活性较低但特异性高，适合需保留细胞膜完整性的实验。其多酶组分可协同分解结缔组织中的蛋白多糖复合物，促进组织解离。
解决实验中的关键问题
1. 组织解离效率低：针对致密结缔组织，胶原酶可高效降解胶原网络，释放单细胞悬液。
2. 细胞活性损伤：温和的酶活性减少细胞膜蛋白破坏，提高原代细胞存活率。
3. 背景杂质高：过滤工艺去除菌体及大分子杂质，降低实验干扰。
4. 消化时间不可控：通过调节浓度（0.5-2 mg/mL）和温度（37°C）精准控制消化进程。
使用方法
1. 缓冲液配制：
• 推荐使用含Ca²⁺、Mg²⁺的Hank’s平衡盐溶液（HBSS），如：
HBSS (1×) + 3 mM CaCl₂ + 0.3 mM MgCl₂
• 避免含EDTA或高浓度DTT的缓冲液，以免抑制酶活性。
2. 酶溶液制备：
• 称取1 mg冻干粉，溶于1 mL预冷缓冲液中，涡旋混匀。
• 0.22 μm滤膜过滤除菌，分装后-20°C保存。
3. 组织消化流程：
• 步骤1：将组织剪碎至1-3 mm³，用PBS清洗3次。
• 步骤2：加入含胶原酶的缓冲液（推荐浓度0.5-1 mg/mL），37°C孵育1-4小时，水平摇床（100 rpm）促进均匀消化。
• 步骤3：每15分钟轻摇一次，观察组织解离程度。
• 步骤4：消化完成后，用含血清的培养液终止反应（血清中和酶活性）。
• 步骤5：4°C离心（500×g, 5分钟），弃上清，重悬细胞于培养基中。
4. 关键参数优化：
• 浓度调整：硬组织（如肝脏）可提高至2 mg/mL，软组织（如脂肪）建议0.5 mg/mL。
• 时间控制：肿瘤组织通常需2-3小时，肝脏灌注实验需1-2小时。
常见问题与解决方案
问题1：消化后细胞存活率低
• 原因：酶浓度过高或消化时间过长。
• 解决：降低浓度至0.2-0.5 mg/mL，缩短消化时间至1小时内。
问题2：背景杂质多
• 原因：酶活性不足或组织块过大。
• 解决：增加酶浓度至1.5 mg/mL，预冷缓冲液中预孵育组织块30分钟。
问题3：酶活性下降快
• 原因：反复冻融或储存温度不当。
• 解决：分装后-80°C冻存，使用前复溶，避免多次冻融。
问题4：消化后细胞团块残留
• 原因：胶原酶与组织接触不充分。
• 解决：使用水平摇床（100-150 rpm）促进混合，或加入0.1% DNase I减少DNA粘性。
问题5：实验重现性差
• 原因：酶批次差异或缓冲液成分波动。
• 解决：每批次验证活性，固定缓冲液配方。
免责声明
1. 本产品为科研用途试剂，不可用于临床诊断或治疗。
2. 使用前需阅读并遵守安全操作规程，操作时佩戴手套及护目镜。
3. 产品性能可能因储存条件不当或超期使用而下降，建议定期验证活性。
4. 本说明书内容基于现有实验数据，具体实验参数需根据实际需求优化。
5. 本文内容基于品牌公开资料及行业常规信息整理，仅个人观点，具体以品牌信息文档为准。
关键词：Worthington胶原蛋白酶Type3、原代细胞分离酶、冻干粉酶制剂、Type3胶原酶、细胞解离酶、肿瘤组织消化、器官灌注实验、低损伤细胞分离、高纯度胶原酶、科研级酶制剂、胶原酶Type3应用、细胞培养酶、冻干酶保存、酶活性稳定、多酶协同解离、HBSS缓冲液配方、细胞活性保护、实验室常用酶、Worthington生物酶、胶原酶技术参数
⚡Worthington热卖产品
|
货号 |
品名 |
规格 |
品牌 |
|
LS004176,CAS：9001.12.1, |
Collagenase, Type 2 (CLS-2)2型胶原酶 |
1gm |
Worthington-biochem |
|
LS004196,原始货号：LS004200,CAS：9001.12.1 |
Collagenase Type 1 |
1gm |
Worthington-biochem |
|
LS004188,CAS：9001.12.1, |
Collagenase, Type 4 |
1gm |
Worthington-biochem |
|
LS004194,CAS：9001.12.1, |
Collagenase Type 1 (CLS-1) |
100mg |
Worthington-biochem |
|
LS004174,CAS：9001.12.1, |
Collagenase, Type 2 |
100mg |
Worthington-biochem |
|
LS004186,CAS：9001.12.1, |
Collagenase, Type 4 |
100mg |
Worthington-biochem |
|
LS004182,CAS：9001.12.1, |
Collagese, Type 3 |
1gm |
Worthington-biochem |
|
ls003126,CAS：9001.73.4, |
Papain, Suspension |
100mg |
Worthington-biochem |
|
LK003150,原始货号：LK003153, |
Papain Dissociation System |
1bx |
Worthington-biochem |
|
LS003119,CAS：9001.73.4, |
Papain, Lyophilized |
100mg |
Worthington-biochem |
|
LS004180,CAS：9001.12.1, |
Collagenase, Type 3 |
100mg |
Worthington-biochem |
|
LS003740,CAS：9002.07.7, |
Trypsin, TPCK Treated |
100mg |
Worthington-biochem |
|
LS002139,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS002292,CAS：39445.21.1, |
Elastase Lyophilized |
100mg |
Worthington-biochem |
|
LK003178,CAS：9001.73.4, |
PDS Kit Papain Vial |
5vi |
Worthington-biochem |
|
LS002279,CAS：39445.21.1, |
Elastase, Suspension (ES) |
100mg |
Worthington-biochem |
|
LS004177,CAS：9001.12.1, |
Collagese, Type 2 |
5gm |
Worthington-biochem |
|
LS002006,CAS：9003.98.9, |
Deoxyribonuclease I |
20mg |
Worthington-biochem |
|
LS002007,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS004189,CAS：9001.12.1, |
Collagenase, Type 4 |
5 gm |
Worthington-biochem |
|
LK003176,原始货号：LK003178,CAS：9001.73.4 |
PDS Kit Papain Vial (PAP2) |
1 vi |
Worthington-biochem |
|
LS002140,CAS：9003.98.9, |
Deoxyribonuclease I |
1 gm |
Worthington-biochem |
|
LS003926,CAS：9025.82.5, |
Phosphodiesterase I |
100un |
Worthington-biochem |
|
LK003182,CAS：9035.81.8/9048.46.8, |
PDS Kit Inhibitor Vial (OI-BSA) |
1 vi |
Worthington-biochem |
|
LS05006,CAS：9068.38.6, |
Reverse Transcriptase, Recombinant, HIV |
5x200 un |
Worthington-biochem |
|
LS002594,CAS：3726.33.3, |
Hyaluronidase |
50ku |
Worthington-biochem |
|
LS004106,CAS：9001.12.1/42613.33.2, |
STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free |
50mg |
Worthington-biochem |
|
LS02100,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
10mg |
Worthington-biochem |
|
LS002290,CAS：39445.21.1, |
Elastase, Lyophilized |
25mg |
Worthington-biochem |
|
LK003170,原始货号：LK003172,CAS：9003.98.9 |
DNase Vial (D2) (D2 |
1 vi |
Worthington-biochem |
|
LS003570,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
100mg |
Worthington-biochem |
|
LS003608,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
1mg |
Worthington-biochem |
|
LS002274,CAS：39445.21.1, |
Elastase, Suspension |
25mg |
Worthington-biochem |
|
LS003127,CAS：9001.73.4, |
Papain, Suspension |
1 gm |
Worthington-biochem |
|
LS003086,CAS：9035.81.8, |
Trypsin Inhbitor, Ovomucoid |
2 gm |
Worthington-biochem |
|
LS003118,CAS：9001.73.4, |
Papain, Lyophilized |
25mg |
Worthington-biochem |
|
LS004212,CAS：9001.12.1, |
Collagenase, Type 4, Filtered Store at 2-8°C. |
5x50mg |
Worthington-biochem |
|
LS004200,CAS：9001.12.1, |
Collagenase, Type 1 |
Bulk |
Worthington-biochem |
|
LS005305,CAS：9025.24.5, |
Carboxypeptidase B |
5mg |
Worthington-biochem |
|
LS004179,CAS：9001.12.1, |
Collagese, Type 2 |
Bulk |
Worthington-biochem |
|
LS004197,CAS：9001.12.1, |
Collagenase Type 1 |
5 gm |
Worthington-biochem |
|
LS003120,CAS：9001.73.4, |
Papain, Lyophilized |
1 gm |
Worthington-biochem |
|
LS004191,CAS：9001.12.1, |
Collagenase, Type 4 |
500g |
Worthington-biochem |
|
LS006344,CAS：9003.98.9, |
Deoxyribonuclease I, RNase & Protease Free, Solution |
500 un |
Worthington-biochem |
|
LS004798,CAS：9013.53.0, |
Nuclease, Micrococcal |
45 ku |
Worthington-biochem |
|
LS02104,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
50mg |
Worthington-biochem |
|
LS003124,CAS：9001.73.4, |
Papain, Suspension |
25mg |
Worthington-biochem |
|
LS003571,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
1 gm |
Worthington-biochem |
|
LS004520,CAS：9028.79.9, |
Galactose Oxidase |
150 un |
Worthington-biochem |
|
LS004522,CAS：9028.79.9, |
Galactose Oxidase |
450 un |
Worthington-biochem |
|
LS001070,CAS：9031-72-5, |
Alcohol Dehydrogese, Lyophilized |
1gm |
Worthington-biochem |
|
LS002138,CAS：9003.98.9, |
Deoxyribonuclease I |
25mg |
Worthington-biochem |
|
LS002592,CAS：3726.33.3, |
Hyaluronidase |
300ku |
Worthington-biochem |
|
LS003793,CAS：9002.10.2, |
Polyphenol Oxidase (Tyrosinase) |
500 ku |
Worthington-biochem |
|
LS004524,CAS：9028.79.9, |
Galactose Oxidase |
1 ku |
Worthington-biochem |
|
LS003605,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
5mg |
Worthington-biochem |
|
LK003163,, |
Papain Dissociation System, Without EBSS |
3 bx |
Worthington-biochem |
|
LS005275,CAS：9001.12.1, |
Collagenase, Purified |
4ku |
Worthington-biochem |
|
LS003741,CAS：9002.07.7, |
Trypsin, TPCK Treated (TRTPCK) |
500mg |
Worthington-biochem |
|
LS05003,CAS：9068.38.6, |
everse Transcriptase Recombinant HIV |
200 un |
Worthington-biochem |
|
LS02111,CAS：42613.33.2, |
Neutral Protease, Partially Purified |
5gm |
Worthington-biochem |
|
LS003587,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
1 gm |
Worthington-biochem |
|
LS006323（500ku）,, |
Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade |
500ku |
Worthington-biochem |
|
LS002933,CAS：12650.88.3, |
Lysozyme, Purified, Salt Free |
5 gm |
Worthington-biochem |
|
LS006123,CAS：9001.78.9, |
Phosphatase, Alkaline, Purified |
25mg |
Worthington-biochem |
|
LS006363,CAS：39445.21.1, |
Elastase, Purified |
5mg |
Worthington-biochem |
|
LS005282,CAS：9001.12.1, |
Collagenase, Type 5 |
1gm |
Worthington-biochem |
|
LS02122,CAS：9002.07.7, |
Trypsin, Modified, SequENZ® Sequencing Grade |
4x100ug |
Worthington-biochem |
|
LS003744,CAS：9002.07.7, |
Trypsin, TPCK Treated |
1 gm |
Worthington-biochem |
|
LS005477,CAS：3726.33.3, |
Hyaluronidase, Purified |
5ku |
Worthington-biochem |
|
LS003589,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
10gm |
Worthington-biochem |
|
LS001141,CAS：9001.77.8, |
Phosphatase, Acid |
1gm |
Worthington-biochem |
|
LS004185,CAS：9001.12.1, |
Collagenase, Type 3 |
500g |
Worthington-biochem |
|
LS003602,CAS：9068.54.6, |
Phosphodiesterase II |
25un |
Worthington-biochem |
|
LS01492,CAS：9026.12.4, |
Ribonuclease T1, Chromatographically Purif., Lyophilized |
2500ku |
Worthington-biochem |
|
LS001656,CAS：9007-34-5, |
Collagen |
10 gm |
Worthington-biochem |
|
LS002106,CAS：9007.49.2, |
Deoxyribonucleic Acid, Calf Thymus |
1 gm |
Worthington-biochem |
|
LS001334,CAS：9004.07.3, |
Chymotrypsin, Alpha, 1X |
10 gm |
Worthington-biochem |
|
LS004780,CAS：9001.67.6, |
Neuraminidase |
10mg |
Worthington-biochem |
|
LS003887,CAS：9002.13.5, |
Urease |
10 gm |
Worthington-biochem |
|
LK003153,, |
Papain Dissociation System |
3bx |
Worthington-biochem |
|
LS001263,CAS：9001.03.0, |
Carbonic Anhydrase |
250mg |
Worthington-biochem |
|
LS004154,CAS：9001.12.1, |
Collagenase, Type A, Animal Free |
1gm |
Worthington-biochem |
|
LS004081,CAS：9001.78.9, |
Phosphatase, Alkaline |
10mg |
Worthington-biochem |
|
LK003172,CAS：9003.98.9, |
DNase Vial (D2) |
5 vi |
Worthington-biochem |
|
LS002764,CAS：9000.89.9, |
Amino Acid Oxidase, L- |
5mg |
Worthington-biochem |
|
LS003981,CAS：9001.50.7, |
Glucose-6-Phosphate Dehydrogenase, Lyophilized |
1 ku |
Worthington-biochem |
|
LS004326-100ku,, |
Diaphorase |
100 ku |
Worthington-biochem |
|
LK003188,, |
PDS Kit, EBSS Via |
1vi |
Worthington-biochem |
|
LS004759,CAS：9001.67.6, |
Neuraminidase, Purified |
5 un |
Worthington-biochem |
|
LS005304,CAS：9025.24.5, |
Carboxypeptidase B |
50mg |
Worthington-biochem |
|
LS006355,CAS：9003.98.9, |
Deoxyribonuclease I, Recombinant, Solution |
5x2 ku |
Worthington-biochem |
|
LS006365,CAS：39445.21.1, |
Elastase, Purified |
20mg |
Worthington-biochem |
|
LK003300,, |
Neonatal Cardiomyocyte Isolation System |
1 kt |
Worthington-biochem |
|
LS02123,CAS：9002.07.7, |
Trypsin, Modified, Sequencing Grade |
1mg |
Worthington-biochem |
|
LS002976,CAS：84195.52.8, |
Mucin |
500mg |
Worthington-biochem |
|
LS01502,CAS：37278.25.4, |
Ribonuclease T2, Recombinant |
250ku |
Worthington-biochem |
|
LS002512,, |
Hexokinase, Lyophilized |
10 ku |
Worthington-biochem |
|
LS004206,CAS：9001.12.1, |
Collagenase, Type 3, Filtered |
50mg |
Worthington-biochem |
|
LS006308,CAS：9003.98.9, |
Amino Acid Oxidase, D- |
25mg |
Worthington-biochem |