13530144996
Collagese, Type 1
货号:LS004196
品名:Collagese, Type 1
品牌:Worthington-biochem
规格:1g
价格:请询价
货期:现货
关键词:
Worthington胶原酶Type 1、原代细胞分离、生物制药、组织解离、酶活性平衡、流式细胞术、单细胞测序、实验室耗材、细胞培养污染控制、ISO认证酶、哺乳动物组织消化、科研试剂、生物技术公司、制药企业诊断、病理样本处理、细胞治疗、靶向细胞分离、酶解效率提升、Worthington代理
产品详情介绍
一、产品基础信息
货号：LS004196
英文名：Collagenase Type 1
中文名：胶原酶I型
品牌：Worthington-biochem
规格：1克（可定制其他规格）
来源：溶组织梭菌（Clostridium histolyticum）
酶活性：含胶原酶、酪蛋白酶、梭菌蛋白酶及胰蛋白酶活性，平衡的酶谱设计使其适用于多种组织解离场景。
储存条件：冻干粉保存于4°C，复溶后建议分装保存于-20°C或-80°C，避免反复冻融。
二、工作原理
Collagenase Type 1通过特异性水解胶原蛋白的三股螺旋结构，优先切割Pro-X-Gly-Pro序列中的中性氨基酸（如甘氨酸）与中性氨基酸之间的肽键，从而降解天然胶原纤维。其复合酶活性包括：
1. 胶原酶活性：分解胶原蛋白，破坏细胞外基质；
2. 酪蛋白酶活性：辅助消化酪蛋白，增强组织渗透性；
3. 梭菌蛋白酶活性：降解细胞间连接蛋白；
4. 胰蛋白酶活性：促进细胞分离，减少细胞团块残留。
该酶的协同作用可高效解离肝脏、肺、脂肪等结缔组织丰富的器官，同时保留细胞膜完整性。
三、解决实验中的核心问题
1. 组织解离效率低：
• 针对肝脏、肺等纤维化组织，传统机械分离法易损伤细胞活性。Collagenase Type 1通过酶解作用实现温和解离，显著提高活细胞得率。
2. 原代细胞分离困难：
• 在乳腺、肾上腺等组织中，胶原酶可靶向分解基底膜，释放单细胞悬液，适用于流式细胞术、单细胞测序等下游实验。
3. 细胞培养污染风险：
• 低胰酶活性设计减少对细胞膜蛋白的损伤，降低培养过程中因蛋白酶残留导致的细胞凋亡。
4. 批次间稳定性差：
• Worthington通过ISO 9001认证的标准化生产流程，确保每批次酶活性一致性，避免实验结果波动。
四、产品优势
1. 高纯度与活性平衡：
• 采用先进的纯化技术，去除金属蛋白酶等杂质，保留核心酶活，适用于敏感细胞类型（如胰岛细胞）。
2. 广泛的应用兼容性：
• 已验证适用于哺乳动物（人、小鼠、大鼠等）的28种组织类型，包括心脏、骨、脂肪等。
3. 严格质量控制：
• 每批次检测胶原酶、酪蛋白酶活性，提供活性报告，确保实验可重复性。
4. 灵活的规格选择：
• 提供100 mg至5克多种包装，满足从实验室研究到中试生产的需求。
五、客户群体
1. 科研机构：
• 高校、研究所用于原代细胞培养、类器官构建及疾病模型开发。
2. 生物技术公司：
• 细胞治疗、疫苗生产等领域需高活性胶原酶支持规模化生产。
3. 制药企业：
• 药物筛选中用于靶细胞分离，如肿瘤微环境研究。
4. 诊断实验室：
• 病理样本处理中分离特定细胞亚群，提升检测准确性。
六、订货信息
• 认证保障：正品瓶口塑封为白底蓝字45°倾斜，标签底色纯白，字体清晰无毛边。
• 技术支持：提供酶活性验证方案及组织解离参数优化指南。
七、常见问题与解答
1. Q：Collagenase Type 1与Type 2有何区别？
• A：Type 1蛋白酶活性更低，适合上皮、肝脏等脆弱组织；Type 2含更高梭菌蛋白酶活性，适用于心脏、肌肉等致密组织。
2. Q：酶活性单位如何定义？
• A：1单位定义为37°C、pH 7.5下5小时释放1 μmol L-亮氨酸等效物。
3. Q：复溶后保存期限？
• A：建议分装后-80°C短期保存，长期存储需避免反复冻融。
4. Q：如何避免假货风险？
• A：通过官方渠道购买，核对瓶口塑封、标签印刷及授权书。
关键词：Worthington胶原酶Type 1、原代细胞分离、生物制药、组织解离、酶活性平衡、流式细胞术、单细胞测序、实验室耗材、细胞培养污染控制、ISO认证酶、哺乳动物组织消化、科研试剂、生物技术公司、制药企业诊断、病理样本处理、细胞治疗、靶向细胞分离、酶解效率提升、Worthington代理、上海起发实验试剂
（注：本文内容基于品牌公开资料及行业常规信息整理，具体以品牌信息文档为准。）
⚡Worthington热卖产品
|
货号 |
品名 |
规格 |
品牌 |
|
LS004176,CAS：9001.12.1, |
Collagenase, Type 2 (CLS-2)2型胶原酶 |
1gm |
Worthington-biochem |
|
LS004196,原始货号：LS004200,CAS：9001.12.1 |
Collagenase Type 1 |
1gm |
Worthington-biochem |
|
LS004188,CAS：9001.12.1, |
Collagenase, Type 4 |
1gm |
Worthington-biochem |
|
LS004194,CAS：9001.12.1, |
Collagenase Type 1 (CLS-1) |
100mg |
Worthington-biochem |
|
LS004174,CAS：9001.12.1, |
Collagenase, Type 2 |
100mg |
Worthington-biochem |
|
LS004186,CAS：9001.12.1, |
Collagenase, Type 4 |
100mg |
Worthington-biochem |
|
LS004182,CAS：9001.12.1, |
Collagese, Type 3 |
1gm |
Worthington-biochem |
|
ls003126,CAS：9001.73.4, |
Papain, Suspension |
100mg |
Worthington-biochem |
|
LK003150,原始货号：LK003153, |
Papain Dissociation System |
1bx |
Worthington-biochem |
|
LS003119,CAS：9001.73.4, |
Papain, Lyophilized |
100mg |
Worthington-biochem |
|
LS004180,CAS：9001.12.1, |
Collagenase, Type 3 |
100mg |
Worthington-biochem |
|
LS003740,CAS：9002.07.7, |
Trypsin, TPCK Treated |
100mg |
Worthington-biochem |
|
LS002139,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS002292,CAS：39445.21.1, |
Elastase Lyophilized |
100mg |
Worthington-biochem |
|
LK003178,CAS：9001.73.4, |
PDS Kit Papain Vial |
5vi |
Worthington-biochem |
|
LS002279,CAS：39445.21.1, |
Elastase, Suspension (ES) |
100mg |
Worthington-biochem |
|
LS004177,CAS：9001.12.1, |
Collagese, Type 2 |
5gm |
Worthington-biochem |
|
LS002006,CAS：9003.98.9, |
Deoxyribonuclease I |
20mg |
Worthington-biochem |
|
LS002007,CAS：9003.98.9, |
Deoxyribonuclease I |
100mg |
Worthington-biochem |
|
LS004189,CAS：9001.12.1, |
Collagenase, Type 4 |
5 gm |
Worthington-biochem |
|
LK003176,原始货号：LK003178,CAS：9001.73.4 |
PDS Kit Papain Vial (PAP2) |
1 vi |
Worthington-biochem |
|
LS002140,CAS：9003.98.9, |
Deoxyribonuclease I |
1 gm |
Worthington-biochem |
|
LS003926,CAS：9025.82.5, |
Phosphodiesterase I |
100un |
Worthington-biochem |
|
LK003182,CAS：9035.81.8/9048.46.8, |
PDS Kit Inhibitor Vial (OI-BSA) |
1 vi |
Worthington-biochem |
|
LS05006,CAS：9068.38.6, |
Reverse Transcriptase, Recombinant, HIV |
5x200 un |
Worthington-biochem |
|
LS002594,CAS：3726.33.3, |
Hyaluronidase |
50ku |
Worthington-biochem |
|
LS004106,CAS：9001.12.1/42613.33.2, |
STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free |
50mg |
Worthington-biochem |
|
LS02100,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
10mg |
Worthington-biochem |
|
LS002290,CAS：39445.21.1, |
Elastase, Lyophilized |
25mg |
Worthington-biochem |
|
LK003170,原始货号：LK003172,CAS：9003.98.9 |
DNase Vial (D2) (D2 |
1 vi |
Worthington-biochem |
|
LS003570,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
100mg |
Worthington-biochem |
|
LS003608,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
1mg |
Worthington-biochem |
|
LS002274,CAS：39445.21.1, |
Elastase, Suspension |
25mg |
Worthington-biochem |
|
LS003127,CAS：9001.73.4, |
Papain, Suspension |
1 gm |
Worthington-biochem |
|
LS003086,CAS：9035.81.8, |
Trypsin Inhbitor, Ovomucoid |
2 gm |
Worthington-biochem |
|
LS003118,CAS：9001.73.4, |
Papain, Lyophilized |
25mg |
Worthington-biochem |
|
LS004212,CAS：9001.12.1, |
Collagenase, Type 4, Filtered Store at 2-8°C. |
5x50mg |
Worthington-biochem |
|
LS004200,CAS：9001.12.1, |
Collagenase, Type 1 |
Bulk |
Worthington-biochem |
|
LS005305,CAS：9025.24.5, |
Carboxypeptidase B |
5mg |
Worthington-biochem |
|
LS004179,CAS：9001.12.1, |
Collagese, Type 2 |
Bulk |
Worthington-biochem |
|
LS004197,CAS：9001.12.1, |
Collagenase Type 1 |
5 gm |
Worthington-biochem |
|
LS003120,CAS：9001.73.4, |
Papain, Lyophilized |
1 gm |
Worthington-biochem |
|
LS004191,CAS：9001.12.1, |
Collagenase, Type 4 |
500g |
Worthington-biochem |
|
LS006344,CAS：9003.98.9, |
Deoxyribonuclease I, RNase & Protease Free, Solution |
500 un |
Worthington-biochem |
|
LS004798,CAS：9013.53.0, |
Nuclease, Micrococcal |
45 ku |
Worthington-biochem |
|
LS02104,CAS：42613.33.2, |
Neutral Protease (Dispase), Purified |
50mg |
Worthington-biochem |
|
LS003124,CAS：9001.73.4, |
Papain, Suspension |
25mg |
Worthington-biochem |
|
LS003571,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean, Purified |
1 gm |
Worthington-biochem |
|
LS004520,CAS：9028.79.9, |
Galactose Oxidase |
150 un |
Worthington-biochem |
|
LS004522,CAS：9028.79.9, |
Galactose Oxidase |
450 un |
Worthington-biochem |
|
LS001070,CAS：9031-72-5, |
Alcohol Dehydrogese, Lyophilized |
1gm |
Worthington-biochem |
|
LS002138,CAS：9003.98.9, |
Deoxyribonuclease I |
25mg |
Worthington-biochem |
|
LS002592,CAS：3726.33.3, |
Hyaluronidase |
300ku |
Worthington-biochem |
|
LS003793,CAS：9002.10.2, |
Polyphenol Oxidase (Tyrosinase) |
500 ku |
Worthington-biochem |
|
LS004524,CAS：9028.79.9, |
Galactose Oxidase |
1 ku |
Worthington-biochem |
|
LS003605,CAS：66676.43.5, |
Protease, S. Aureus (Endoproteinase Glu-C) |
5mg |
Worthington-biochem |
|
LK003163,, |
Papain Dissociation System, Without EBSS |
3 bx |
Worthington-biochem |
|
LS005275,CAS：9001.12.1, |
Collagenase, Purified |
4ku |
Worthington-biochem |
|
LS003741,CAS：9002.07.7, |
Trypsin, TPCK Treated (TRTPCK) |
500mg |
Worthington-biochem |
|
LS05003,CAS：9068.38.6, |
everse Transcriptase Recombinant HIV |
200 un |
Worthington-biochem |
|
LS02111,CAS：42613.33.2, |
Neutral Protease, Partially Purified |
5gm |
Worthington-biochem |
|
LS003587,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
1 gm |
Worthington-biochem |
|
LS006323（500ku）,, |
Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade |
500ku |
Worthington-biochem |
|
LS002933,CAS：12650.88.3, |
Lysozyme, Purified, Salt Free |
5 gm |
Worthington-biochem |
|
LS006123,CAS：9001.78.9, |
Phosphatase, Alkaline, Purified |
25mg |
Worthington-biochem |
|
LS006363,CAS：39445.21.1, |
Elastase, Purified |
5mg |
Worthington-biochem |
|
LS005282,CAS：9001.12.1, |
Collagenase, Type 5 |
1gm |
Worthington-biochem |
|
LS02122,CAS：9002.07.7, |
Trypsin, Modified, SequENZ® Sequencing Grade |
4x100ug |
Worthington-biochem |
|
LS003744,CAS：9002.07.7, |
Trypsin, TPCK Treated |
1 gm |
Worthington-biochem |
|
LS005477,CAS：3726.33.3, |
Hyaluronidase, Purified |
5ku |
Worthington-biochem |
|
LS003589,CAS：9035.81.8, |
Trypsin Inhibitor, Soybean |
10gm |
Worthington-biochem |
|
LS001141,CAS：9001.77.8, |
Phosphatase, Acid |
1gm |
Worthington-biochem |
|
LS004185,CAS：9001.12.1, |
Collagenase, Type 3 |
500g |
Worthington-biochem |
|
LS003602,CAS：9068.54.6, |
Phosphodiesterase II |
25un |
Worthington-biochem |
|
LS01492,CAS：9026.12.4, |
Ribonuclease T1, Chromatographically Purif., Lyophilized |
2500ku |
Worthington-biochem |
|
LS001656,CAS：9007-34-5, |
Collagen |
10 gm |
Worthington-biochem |
|
LS002106,CAS：9007.49.2, |
Deoxyribonucleic Acid, Calf Thymus |
1 gm |
Worthington-biochem |
|
LS001334,CAS：9004.07.3, |
Chymotrypsin, Alpha, 1X |
10 gm |
Worthington-biochem |
|
LS004780,CAS：9001.67.6, |
Neuraminidase |
10mg |
Worthington-biochem |
|
LS003887,CAS：9002.13.5, |
Urease |
10 gm |
Worthington-biochem |
|
LK003153,, |
Papain Dissociation System |
3bx |
Worthington-biochem |
|
LS001263,CAS：9001.03.0, |
Carbonic Anhydrase |
250mg |
Worthington-biochem |
|
LS004154,CAS：9001.12.1, |
Collagenase, Type A, Animal Free |
1gm |
Worthington-biochem |
|
LS004081,CAS：9001.78.9, |
Phosphatase, Alkaline |
10mg |
Worthington-biochem |
|
LK003172,CAS：9003.98.9, |
DNase Vial (D2) |
5 vi |
Worthington-biochem |
|
LS002764,CAS：9000.89.9, |
Amino Acid Oxidase, L- |
5mg |
Worthington-biochem |
|
LS003981,CAS：9001.50.7, |
Glucose-6-Phosphate Dehydrogenase, Lyophilized |
1 ku |
Worthington-biochem |
|
LS004326-100ku,, |
Diaphorase |
100 ku |
Worthington-biochem |
|
LK003188,, |
PDS Kit, EBSS Via |
1vi |
Worthington-biochem |
|
LS004759,CAS：9001.67.6, |
Neuraminidase, Purified |
5 un |
Worthington-biochem |
|
LS005304,CAS：9025.24.5, |
Carboxypeptidase B |
50mg |
Worthington-biochem |
|
LS006355,CAS：9003.98.9, |
Deoxyribonuclease I, Recombinant, Solution |
5x2 ku |
Worthington-biochem |
|
LS006365,CAS：39445.21.1, |
Elastase, Purified |
20mg |
Worthington-biochem |
|
LK003300,, |
Neonatal Cardiomyocyte Isolation System |
1 kt |
Worthington-biochem |
|
LS02123,CAS：9002.07.7, |
Trypsin, Modified, Sequencing Grade |
1mg |
Worthington-biochem |
|
LS002976,CAS：84195.52.8, |
Mucin |
500mg |
Worthington-biochem |
|
LS01502,CAS：37278.25.4, |
Ribonuclease T2, Recombinant |
250ku |
Worthington-biochem |
|
LS002512,, |
Hexokinase, Lyophilized |
10 ku |
Worthington-biochem |
|
LS004206,CAS：9001.12.1, |
Collagenase, Type 3, Filtered |
50mg |
Worthington-biochem |
|
LS006308,CAS：9003.98.9, |
Amino Acid Oxidase, D- |
25mg |
Worthington-biochem |